采用生物发光技术测定脂多糖。
Determination of lipopolysaccharide by a bioluminescence technique.
作者信息
Ulitzur S, Yagen B, Rottem S
出版信息
Appl Environ Microbiol. 1979 Apr;37(4):782-4. doi: 10.1128/aem.37.4.782-784.1979.
Abstract
The determination of the lipid A content of bacterial lipopolysaccharide by using a dim mutant of the luminous bacterium Beneckea harveyi is described. The luminous bacteria emitted light upon the addition of an acid hydrolysate of lipopolysaccharide which contained myristic acid, thus making it possible to detect as little as 1 ng of lipopolysaccharide. By converting the 3-OH-myristic acid to myristic acid, it was possible to further increase the detection sensitivity and to establish a basis for a specific and highly sensitive bioassay for the detection of lipopolysaccharide.
摘要
描述了利用哈维贝内克氏发光菌的双突变体测定细菌脂多糖中脂质A含量的方法。当加入含有肉豆蔻酸的脂多糖酸水解产物时，发光细菌会发光，从而能够检测低至1 ng的脂多糖。通过将3-羟基肉豆蔻酸转化为肉豆蔻酸，可以进一步提高检测灵敏度，并为检测脂多糖建立一种特异性高灵敏度生物测定的基础。
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本文引用的文献
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A new method for the extraction of R lipopolysaccharides.一种提取R脂多糖的新方法。
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Thermal regulation of the fatty acid composition of lipopolysaccharides and phospholipids of Proteus mirabilis.
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Myristic acid stimulation of bacterial bioluminescence in "aldehyde" mutants.肉豆蔻酸对“醛”突变体中细菌生物发光的刺激作用。
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