Bolarin D M, Barker K, Fuller G C
Exp Mol Pathol. 1987 Apr;46(2):145-52. doi: 10.1016/0014-4800(87)90060-8.
Serum galactosylhydroxylysyl glucosyltransferase (S-Glu-Gal-Hyl-Tase), liver galactosylhydroxylysyl glucosyltransferase (L-Glu-Gal-Hyl-Tase), liver hydroxylysyl galactosyltransferase (L-Gal-Hyl-Tase), and liver prolyl hydroxylase (L-PH) activities were measured in rats during the development of CCl4-induced cirrhosis (0.2 ml of 33% CCl4 in light mineral oil two times weekly for 10 weeks followed by 6 weeks of no treatment). Serum and liver markers of collagen synthesis increased in a time-dependent manner reaching maximum activity at 6 weeks (S-Glu-Gal-Hyl-Tase, two times; L-PH, two times). These enzyme levels returned to normal during the 4-week recovery period. In a separate 4-week experiment, colchicine (10 micrograms/rat/day) was administered with CCl4. Colchicine prevented the increase in S-Glu-Gal-Hyl-Tase, L-Glu-Gal-Hyl-Tase, and L-Gal-Hyl-Tase induced by CCl4 and resulted in a smaller increase in L-PH. These results demonstrate that S-Glu-Gal-Hyl-Tase elevation occurs following CCl4 because of increased liver collagen synthetic activity and the hepatocellular injury produced by CCl4.
在四氯化碳诱导的肝硬化大鼠模型建立过程中（每周两次腹腔注射0.2 ml 33%四氯化碳的轻质矿物油溶液，共10周，随后6周不进行处理），测定血清半乳糖基羟赖氨酰葡糖基转移酶（S-Glu-Gal-Hyl-Tase）、肝脏半乳糖基羟赖氨酰葡糖基转移酶（L-Glu-Gal-Hyl-Tase）、肝脏羟赖氨酰半乳糖基转移酶（L-Gal-Hyl-Tase）和肝脏脯氨酰羟化酶（L-PH）的活性。血清和肝脏胶原合成标志物呈时间依赖性增加，在第6周达到最大活性（S-Glu-Gal-Hyl-Tase增加两倍；L-PH增加两倍）。在4周的恢复期内，这些酶水平恢复正常。在另一个为期4周的实验中，秋水仙碱（10微克/大鼠/天）与四氯化碳同时给药。秋水仙碱可防止四氯化碳诱导的S-Glu-Gal-Hyl-Tase、L-Glu-Gal-Hyl-Tase和L-Gal-Hyl-Tase活性增加，并使L-PH的增加幅度减小。这些结果表明，四氯化碳诱导后S-Glu-Gal-Hyl-Tase活性升高是由于肝脏胶原合成活性增加以及四氯化碳所致的肝细胞损伤。
