Favari L, Pérez-Alvarez V
Departamento de Farmacología y Toxicologia, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.
Arch Med Res. 1997 Spring;28(1):11-7.
The comparative effects of colchicine (10 micrograms day-1, p.o.) and silymarin (50 mg kg-1, p.o.) each given for 5 days a week on the chronic carbon tetrachloride (CCl4) liver damage were studied. Treatment with CCl4 resulted in a marked reduction of Na+, K+, and Ca2(+)-ATPases in plasma liver membranes as compared to vehicles or either silymarin or colchicine alone. Collagen content in livers of animals treated with CCl4 was increased about four-fold as compared to controls and histological examination of liver samples showed that collagen increase distorted the normal liver architecture. Colchicine or silymarin treatment completely prevented all the changes observed in CCl4-cirrhotic rats (namely, lipid peroxidation, Na+, K+ and Ca(2+)-ATPases), except for liver collagen content which was reduced only 55% as compared with CCl4-treated rats and for alkaline phosphatase and glutamic pyruvic transaminase which still remained above controls. In the CCl4 + silymarin group, the loss of glycogen content was completely prevented. However, when rats were treated with CCl4 + colchicine, liver glycogen content could not be restored. The hepatoprotective effects of colchicine or silymarin were very similar in regard to the prevention of chronic liver damage.
研究了秋水仙碱（每天口服10微克）和水飞蓟宾（每千克体重口服50毫克）每周给药5天对四氯化碳（CCl4）所致慢性肝损伤的比较效果。与溶剂对照组、单独使用水飞蓟宾或秋水仙碱相比，CCl4处理导致血浆肝细胞膜中Na+、K+和Ca2(+)-ATP酶显著减少。与对照组相比，CCl4处理的动物肝脏中胶原蛋白含量增加了约四倍，肝脏样本的组织学检查表明，胶原蛋白增加使正常肝脏结构变形。秋水仙碱或水飞蓟宾处理完全预防了CCl4诱导的肝硬化大鼠中观察到的所有变化（即脂质过氧化、Na+、K+和Ca(2+)-ATP酶），但肝脏胶原蛋白含量仅比CCl4处理的大鼠降低了55%，碱性磷酸酶和谷丙转氨酶仍高于对照组。在CCl4 + 水飞蓟宾组中，糖原含量的损失得到了完全预防。然而，当大鼠用CCl4 + 秋水仙碱处理时，肝脏糖原含量无法恢复。秋水仙碱或水飞蓟宾在预防慢性肝损伤方面的保肝作用非常相似。
