Risteli J, Kivirikko K I
Biochem J. 1976 Aug 15;158(2):361-7. doi: 10.1042/bj1580361.
The relationship between the changes in the four enzyme activities catalysing intracellular post-translational modifications in collagen biosynthesis were studied in rat liver as a function of age and in experimental hepatic injury induced by the administration of dimethylnitrosamine. During aging, relatively large changes were found in prolyl hydroxylase and lysyl hydroxylase activities, whereas only minor changes took place in collagen galactosyltransferase and collagen glucosyltransferase activities. In hepatic injury, the two hydroxylase activities increased earlier and to a larger extent than did the two glycosyltransferase activities, and the largest was found in lysyl hydroxylase activity. The data support previous suggestions that changes in the rate of collagen biosynthesis in the liver cannot be explained simply by a change in the number of collagen-producing cells, but regulation of the enzyme activities existed, so that the two hydroxylase activities altered considerably more than did the two collagen glycosyltransferase activities.
在大鼠肝脏中，研究了胶原生物合成过程中催化细胞内翻译后修饰的四种酶活性变化与年龄的关系，以及二甲基亚硝胺诱导的实验性肝损伤中的变化。在衰老过程中，脯氨酰羟化酶和赖氨酰羟化酶活性发生了相对较大的变化，而胶原半乳糖基转移酶和胶原葡萄糖基转移酶活性仅发生了微小变化。在肝损伤中，两种羟化酶活性比两种糖基转移酶活性更早且更大程度地增加，其中赖氨酰羟化酶活性增加最大。这些数据支持了先前的观点，即肝脏中胶原生物合成速率的变化不能简单地用产生胶原的细胞数量变化来解释，而是存在酶活性的调节，因此两种羟化酶活性的变化比两种胶原糖基转移酶活性的变化大得多。
