DOE Joint Genome Institute, Walnut Creek, CA, USA.
Department of Biology, University of North Carolina, Chapel Hill, NC, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
Cell Host Microbe. 2018 Oct 10;24(4):475-485. doi: 10.1016/j.chom.2018.09.005.
There is a growing appreciation for the important roles microorganisms play in association with plants. Microorganisms are drawn to distinct plant surfaces by the nutrient-rich microenvironment, and in turn some of these colonizing microbes provide mutualistic benefits to their host. The development of plant probiotics to increase crop yield and provide plant resistance against biotic and abiotic stresses, while minimizing chemical inputs, would benefit from a deeper mechanistic understanding of plant-microbe interaction. Technological advances in molecular biology and high-throughput -omics provide stepping stones to the elucidation of critical microbiome gene functions that aid in improving plant performance. Here, we review -omics-based approaches that are propelling forward the current understanding of plant-associated bacterial gene functions, and describe how these technologies have helped unravel key bacterial genes and pathways that mediate pathogenic, beneficial, and commensal host interactions.
人们越来越意识到微生物在与植物相关联的过程中所扮演的重要角色。微生物被营养丰富的微环境吸引到不同的植物表面，而其中一些定植的微生物则为它们的宿主提供互利的好处。为了提高作物产量并提供植物对生物和非生物胁迫的抗性，同时最大限度地减少化学投入，开发植物益生菌将受益于对植物-微生物相互作用的更深入的机制理解。分子生物学和高通量组学技术的进步为阐明有助于改善植物性能的关键微生物组基因功能提供了垫脚石。在这里，我们回顾了基于组学的方法，这些方法推动了人们对植物相关细菌基因功能的现有理解，并描述了这些技术如何帮助揭示介导病原、有益和共生宿主相互作用的关键细菌基因和途径。
