细胞色素b5与细胞色素c相互作用的核磁共振研究。观察到两种蛋白质与[Cr(en)3]3+形成的三元复合物。
NMR study of the interaction between cytochrome b5 and cytochrome c. Observation of a ternary complex formed by the two proteins and [Cr(en)3]3+.
作者信息
Hartshorn R T, Mauk A G, Mauk M R, Moore G R
出版信息
FEBS Lett. 1987 Mar 23;213(2):391-5. doi: 10.1016/0014-5793(87)81528-4.
PMID:3030818
Abstract
The interaction between horse cytochrome c and the tryptic fragment of bovine liver microsomal cytochrome b5 in the absence and presence of [Cr(ethylenediamine)3]Cl3 was studied by 1H NMR spectroscopy. The protein-protein interaction region on cytochrome b5 was found to be different from the [Cr(en)3]3+-binding region. The solvent-exposed propionate-bearing edge of the haem of cytochrome b5 is accessible to [Cr(en)3]3+ in the interprotein complex.
摘要
通过1H NMR光谱研究了在不存在和存在[Cr(乙二胺)3]Cl3的情况下马细胞色素c与牛肝微粒体细胞色素b5胰蛋白酶片段之间的相互作用。发现细胞色素b5上的蛋白质-蛋白质相互作用区域与[Cr(en)3]3+结合区域不同。在蛋白质间复合物中，细胞色素b5血红素的溶剂暴露的含丙酸酯边缘可被[Cr(en)3]3+接近。
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