[促肾上腺皮质激素（ACTH）或人绒毛膜促性腺激素（hCG）对不同年龄大鼠肾上腺皮质酮和孕酮分泌的影响]

[Effects of ACTH or hCG on corticosterone and progesterone secretion from adrenal glands of the rats at various ages].

作者信息

Kumai A, Kawasaki T, Sassa S, Okamoto R

出版信息

Nihon Naibunpi Gakkai Zasshi. 1987 Jan 20;63(1):10-8. doi: 10.1507/endocrine1927.63.1_10.

DOI:10.1507/endocrine1927.63.1_10

Abstract

Effects of ACTH or hCG on the secretion of corticosterone (CB) and progesterone (PRG) from adrenal glands of the female rats at various ages were studied. In in vitro experiment, adrenal gland cells were isolated from fetal, neonatal, immature and adult rats by the method described by us before and were incubated in MEM. We chose to designate the hormones in the medium as hormone secretion. In in vivo experiment, hormones in the serum were determined. In vitro experiment, in which ACTH was added to the medium, indicated that CB was low in the media containing the cells from fetal and neonatal glands compared to those from immature and adult. PRG was elevated by ACTH addition at all of the ages. hCG addition had no effects on both CB and PRG in the media of immature and adult rats. In vivo experiment, in which ACTH was injected s.c., indicated that the response of serum PRG was slightly earlier than the CB response and peaked with a subsequent decline in immature rats. The results suggest that adrenal gland responds to ACTH from fetal age and secrets PRG, but little of CB in fetal age.

摘要

研究了促肾上腺皮质激素（ACTH）或人绒毛膜促性腺激素（hCG）对不同年龄雌性大鼠肾上腺皮质酮（CB）和孕酮（PRG）分泌的影响。在体外实验中，按照我们之前描述的方法从胎儿、新生、未成熟和成年大鼠中分离肾上腺细胞，并在最低限度基本培养基（MEM）中培养。我们选择将培养基中的激素称为激素分泌。在体内实验中，测定血清中的激素。体外实验中，向培养基中添加ACTH，结果表明，与未成熟和成年大鼠的肾上腺细胞相比，含有胎儿和新生大鼠肾上腺细胞的培养基中CB含量较低。在所有年龄段，添加ACTH均可使PRG升高。在未成熟和成年大鼠的培养基中添加hCG对CB和PRG均无影响。在体内实验中，皮下注射ACTH，结果表明，未成熟大鼠血清PRG的反应略早于CB反应，且先达到峰值，随后下降。结果表明，肾上腺从胎儿期就对ACTH有反应并分泌PRG，但在胎儿期分泌的CB很少。