利用固定化氰酸盐水解酶在磁性多壁碳纳米管上同时去除废水中的重金属和氰酸盐。
Simultaneous removal of heavy metals and cyanate in a wastewater sample using immobilized cyanate hydratase on magnetic-multiwall carbon nanotubes.
机构信息
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa.
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa.
出版信息
J Hazard Mater. 2019 Feb 5;363:73-80. doi: 10.1016/j.jhazmat.2018.07.116. Epub 2018 Sep 13.
Global environmental problems allied with waste management require novel approaches for the simultaneous removal of heavy metals and other associated compounds including cyanate. In this study, iron-oxide filled multi-walled carbon nanotubes (m-MWCNTs) were successfully synthesized and characterized by field emission gun scanning electron microscopy (FEGSEM), high-resolution transmission electron microscopy (HRTEM) and X-ray diffraction (XRD). The m-MWCNTs were amino-functionalized for the covalent immobilization of a recombinant cyanate hydratase (rTl-Cyn), and were characterized by fourier transform infrared (FTIR) spectroscopy. The immobilized rTl-Cyn on the m-MWCNTs (m-MWCNT-rTl-Cyn) had long term storage stability and showed great potential towards cyanate biodegradability. We found that m-MWCNT-rTl-Cyn retained >94% of the initial activity even after 10 repeated cycles of bio-catalysis. Strikingly, the m-MWCNT-rTl-Cyn simultaneously reduced the concentration of chromium (Cr), iron (Fe), lead (Pb) and copper (Cu) by 39.31, 35.53, 34.48 and 29.63%, respectively as well as the concentration of cyanate by ≥84%, in a synthetic wastewater sample.
全球环境问题与废物管理相结合，需要新的方法来同时去除重金属和其他相关化合物，包括氰酸盐。在这项研究中，成功地合成了氧化铁填充的多壁碳纳米管（m-MWCNTs），并通过场发射枪扫描电子显微镜（FEGSEM）、高分辨率透射电子显微镜（HRTEM）和 X 射线衍射（XRD）进行了表征。m-MWCNTs 被氨基官能化，用于共价固定重组氰酸盐水解酶（rTl-Cyn），并通过傅里叶变换红外（FTIR）光谱进行了表征。固定在 m-MWCNTs 上的 rTl-Cyn（m-MWCNT-rTl-Cyn）具有长期储存稳定性，并显示出对氰酸盐生物降解性的巨大潜力。我们发现，即使经过 10 次重复的生物催化循环，m-MWCNT-rTl-Cyn 仍保留了超过 94%的初始活性。引人注目的是，m-MWCNT-rTl-Cyn 同时将合成废水中的铬（Cr）、铁（Fe）、铅（Pb）和铜（Cu）浓度分别降低了 39.31%、35.53%、34.48%和 29.63%，同时将氰酸盐浓度降低了≥84%。
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