Prabhukarthikeyan Seenichamy Rathinam, Parameswaran Chidambaranathan, Sawant Shraddha Bhaskar, Naveenkumar Ramasamy, Mahanty Arabinda, Keerthana Umapathy, Yadav Manoj Kumar, Anandan Annamalai, Panneerselvam Periyasamy, Bag Manas Kumar, Rath Prakash Chandra
ICAR-National Rice Research Institute, Cuttack 753006, India.
J Fungi (Basel). 2022 Apr 5;8(4):370. doi: 10.3390/jof8040370.
Sheath blight of rice is a destructive disease that could be calamitous to rice cultivation. The significant objective of this study is to contemplate the proteomic analysis of the high virulent and less virulent isolate of using a quantitative LC-MS/MS-based proteomic approach to identify the differentially expressed proteins promoting higher virulence. Across several rice-growing regions in Odisha, Eastern India, 58 isolates were obtained. All the isolates varied in their pathogenicity. The isolate RS15 was found to be the most virulent and RS22 was identified as the least virulent. The PCR amplification confirmed that the RS15 and RS22 belonged to the subgroup of AG1-IA with a specific primer. The proteomic information generated has been deposited in the PRIDE database with PXD023430. The virulent isolate consisted of 48 differentially abundant proteins, out of which 27 proteins had higher abundance, while 21 proteins had lower abundance. The analyzed proteins acquired functionality in fungal development, sporulation, morphology, pathogenicity, detoxification, antifungal activity, essential metabolism and transcriptional activities, protein biosynthesis, glycolysis, phosphorylation and catalytic activities in fungi. A Quantitative Real-Time PCR (qRT-PCR) was used to validate changes in differentially expressed proteins at the mRNA level for selected genes. The abundances of proteins and transcripts were positively correlated. This study provides the role of the proteome in the pathogenicity of AG1-IA in rice and underpins the mechanism behind the pathogen's virulence in causing sheath blight disease.
水稻纹枯病是一种对水稻种植具有毁灭性的病害。本研究的重要目标是采用基于液相色谱-串联质谱的定量蛋白质组学方法，对高毒力和低毒力分离株进行蛋白质组分析，以鉴定促进更高毒力的差异表达蛋白。在印度东部奥里萨邦的几个水稻种植区，获得了58个分离株。所有分离株的致病性各不相同。发现分离株RS15毒力最强，RS22被鉴定为毒力最弱。PCR扩增证实，RS15和RS22属于AG1-IA的 亚组，使用了特异性引物。所产生的蛋白质组学信息已存入PRIDE数据库，编号为PXD023430。高毒力分离株包含48种差异丰富的蛋白质，其中27种蛋白质丰度较高，21种蛋白质丰度较低。所分析的蛋白质在真菌发育、孢子形成、形态、致病性、解毒、抗真菌活性、基本代谢和转录活性、蛋白质生物合成、糖酵解、磷酸化以及真菌中的催化活性等方面具有功能。采用定量实时荧光定量PCR（qRT-PCR）来验证所选基因在mRNA水平上差异表达蛋白的变化。蛋白质和转录本的丰度呈正相关。本研究揭示了蛋白质组在水稻AG1-IA致病性中的作用，并阐明了病原菌在引起纹枯病时毒力背后的机制。
