Hsieh K H, Shu S Y, Lee C S, Chu C T, Yang C S, Chang K J
Gut. 1987 Feb;28(2):117-24. doi: 10.1136/gut.28.2.117.
Lymphokine activated killer cell is a newly described lytic system against a variety of solid tumours and is distinct in several respects from the classic cytolytic T cell and the natural killer systems. This study was conducted to evaluate the lytic activity of lymphokine activated killer cells against fresh autologous and allogeneic, as well as cultured hepatocellular carcinoma cells. Lymphokine activated killer cell was generated by incubating peripheral blood mononuclear cells with various concentrations of recombinant IL-2 (rIL-2, Cetus, USA) for various periods of time. A four hour 51Cr release assay was used to measure cytotoxicity. The results show that fresh and cultured hepatocellular carcinoma cells were only slightly susceptible to natural killer cells. Normal hepatocytes were resistant to lymphokine activated killer-mediated lysis. Lymphokine activated killer cells could be generated from mononuclear cells of hepatocellular carcinoma patients and normal subjects with lytic activity against fresh autologous and allogeneic and cultured hepatocellular carcinoma cells, but lymphokine activated killer cells from the former was less efficient than that from the latter. It is concluded that the adoptive immunotherapy with combined rIL-2 and lymphokine activated killer may be worth trying in early cases of primary hepatocellular carcinoma.
淋巴因子激活的杀伤细胞是一种新描述的针对多种实体瘤的溶细胞系统，在几个方面与经典的细胞毒性T细胞和自然杀伤系统不同。本研究旨在评估淋巴因子激活的杀伤细胞对新鲜自体和异体以及培养的肝癌细胞的溶细胞活性。通过将外周血单个核细胞与不同浓度的重组白细胞介素-2（rIL-2，美国Cetus公司）孵育不同时间来产生淋巴因子激活的杀伤细胞。采用四小时51Cr释放试验来测量细胞毒性。结果表明，新鲜的和培养的肝癌细胞仅对自然杀伤细胞稍有敏感性。正常肝细胞对淋巴因子激活的杀伤细胞介导的裂解具有抗性。肝癌患者和正常受试者的单个核细胞均可产生对新鲜自体、异体及培养的肝癌细胞具有溶细胞活性的淋巴因子激活的杀伤细胞，但前者产生的淋巴因子激活的杀伤细胞的效率低于后者。结论是，联合rIL-2和淋巴因子激活的杀伤细胞的过继免疫疗法在原发性肝癌早期病例中可能值得一试。
