Rosenberg S A, Grimm E A, McGrogan M, Doyle M, Kawasaki E, Koths K, Mark D F
Science. 1984 Mar 30;223(4643):1412-4. doi: 10.1126/science.6367046.
The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization. No functional differences between native and the recombinant interleukin-2 molecules have been detected.
白细胞介素-2基因是从Jurkat细胞系和正常外周血淋巴细胞中分离出来的，当插入大肠杆菌中时，能以高浓度表达。这种白细胞介素-2被纯化至明显的均一性，并在多种体外和体内试验中检测其生物活性。重组淋巴因子支持鼠和人白细胞介素-2依赖细胞系的生长，增强体外鼠和人溶细胞性细胞的生成，并从鼠和人淋巴细胞中产生淋巴因子激活的杀伤细胞。它在小鼠体内的血清半衰期为2至3分钟，在同种异体免疫后能显著增强体内溶细胞性细胞的生成。未检测到天然和重组白细胞介素-2分子之间的功能差异。
