Merluzzi V J, Savage D M, Mertelsmann R, Welte K
Cell Immunol. 1984 Mar;84(1):74-84. doi: 10.1016/0008-8749(84)90078-9.
Murine spleen cells developed into nonspecific cytotoxic cells within 72 hr of culture in the presence of highly purified sources of human interleukin 2. In whole spleen cell cultures, human interleukin 2 generated effector cells which were Thy 1.2+, Lyt 2.2+, resistant to gamma irradiation (1000 R), and capable of lysing both H-2 compatible and incompatible targets. The effector cells generated in this manner were not restricted to classical natural killer cell-sensitive targets. If thymus-derived cells (T cells) were depleted from the spleen cell population before culture with human interleukin 2, the effector cells generated were enriched in effectors capable of lysing natural killer cell-sensitive targets. Interferon was not produced in interleukin 2-stimulated spleen cell cultures. In addition, heterologous antibody to murine gamma-interferon did not abrogate the generation of cytotoxic cells by human interleukin 2. These and additional data suggest that human interleukin 2 is capable of stimulating gamma-irradiation-sensitive Thy 1.2+ cell(s) capable of lysing a variety of target cells regardless of inherent sensitivities to classical natural killer cells. Thy 1.2- cells were also stimulated by human interleukin 2 and lysed only natural killer cell-sensitive targets. Human interleukin 2 caused some Thy 1.2- cells to become susceptible to lysis by anti-Thy 1.2 serum and complement.
在高纯度人白细胞介素2存在的情况下，小鼠脾细胞在培养72小时内发育成非特异性细胞毒性细胞。在全脾细胞培养中，人白细胞介素2产生效应细胞，这些细胞为Thy 1.2 +、Lyt 2.2 +，对γ射线照射（1000伦琴）有抗性，并且能够裂解H-2相容和不相容的靶细胞。以这种方式产生的效应细胞不限于经典自然杀伤细胞敏感的靶细胞。如果在用人类白细胞介素2培养之前从脾细胞群体中去除胸腺来源的细胞（T细胞），则产生的效应细胞富含能够裂解自然杀伤细胞敏感靶细胞的效应细胞。在白细胞介素2刺激的脾细胞培养物中不产生干扰素。此外，抗小鼠γ干扰素的异源抗体不会消除人白细胞介素2产生细胞毒性细胞的能力。这些及其他数据表明，人白细胞介素2能够刺激对γ射线照射敏感的Thy 1.2 +细胞，这些细胞能够裂解各种靶细胞，而不管其对经典自然杀伤细胞的固有敏感性如何。Thy 1.2 -细胞也受到人白细胞介素2的刺激，并且仅裂解自然杀伤细胞敏感的靶细胞。人白细胞介素2使一些Thy 1.2 -细胞易被抗Thy 1.2血清和补体裂解。
