Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, Vigo 36208, Pontevedra, Spain.
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela 15782, A Coruña, Spain.
Food Chem. 2019 Jan 30;272:279-285. doi: 10.1016/j.foodchem.2018.08.031. Epub 2018 Aug 10.
The quantification of species in commercial products is limited by analytical shortcomings, as most of them provide semiquantitative results. An exception is real-time PCR, which can provide quantitative results using hybridization probes. In the present work, this technique has been applied to the absolute, absolute-relative and relative quantification of the most valued hake species in European markets, Merluccius merluccius (European Hake). The best quantification results for this species in binary mixtures with non-target species (Merluccius capensis) and using a species-specific real-time PCR MMER_VIC system was achieved using a relative quantification approach (MLL as reference system). Absolute quantification using the MLL nuclear system has been demonstrated as appropriate for the quantification of the Merluccius genus in food model samples. This study reveals the impact of different reference systems (MLL and HAKE) in the absolute-relative and relative quantification approaches, showing that the nuclear MLL system performed better than the mitochondrial HAKE system.
商业产品中物种的定量受到分析缺陷的限制，因为它们大多数提供半定量结果。实时 PCR 是一个例外，它可以使用杂交探针提供定量结果。在本工作中，该技术已应用于欧洲市场上最有价值的无须鳕物种，即 Merluccius merluccius（欧洲无须鳕）的绝对、绝对相对和相对定量。在与非目标物种（Merluccius capensis）的二元混合物中，以及使用特定物种的实时 PCR MMER_VIC 系统，通过相对定量方法（以 MLL 作为参考系统）获得了该物种的最佳定量结果。使用 MLL 核系统进行绝对定量已被证明适用于食品模型样品中 Merluccius 属的定量。本研究揭示了不同参考系统（MLL 和 HAKE）在绝对相对和相对定量方法中的影响，表明核 MLL 系统的性能优于线粒体 HAKE 系统。
