从新西兰棘皮海胆（Evechinus chloroticus）的壳和刺中提取、结构表征和多羟基萘醌的稳定性。

Extraction, structural characterization and stability of polyhydroxylated naphthoquinones from shell and spine of New Zealand sea urchin (Evechinus chloroticus).

机构信息

Department of Food Science, University of Otago, PO Box 56, Dunedin 9054, New Zealand.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia.

出版信息

Food Chem. 2019 Jan 30;272:379-387. doi: 10.1016/j.foodchem.2018.08.046. Epub 2018 Aug 10.

DOI:10.1016/j.foodchem.2018.08.046

PMID:30309558

Abstract

The extraction of polyhydroxylated naphthoquinone (PHNQ) pigments from the shell and spines of the New Zealand sea urchin Evechinus chloroticus was evaluated using six different macroporous resins as an alternative to using organic solvent extraction alone. Four of the resins evaluated in this study (D4006, D4020, D101 and NKA-9) provided the best extraction of PHNQ pigments in terms of the overall adsorption and desorption of E. chloroticus PHNQ pigments from the resins. Organic solvents alone had a higher yield of PHNQs than the resins. The PHNQ composition was characterised by high-performance liquid chromatography (HPLC) with diode-array detection and mass spectrometry. Five PHNQ compounds (spinochromes E, B, C, A and echinochrome A), and three aminated PHNQ compounds (spinamine E, echinamines A and B) were identified. The pigments were found to be prone to degradation on exposure to light, with the aminated PHNQ pigments being the least stable.

摘要

从新西兰棘皮海胆(Evechinus chloroticus)的壳和刺中提取多羟基萘醌(PHNQ)色素，评估了六种不同的大孔树脂作为替代有机溶剂萃取的方法。在这项研究中评估的四种树脂（D4006、D4020、D101 和 NKA-9）在从树脂中吸附和解析 E. chloroticus PHNQ 色素方面提供了 PHNQ 色素的最佳提取。有机溶剂单独提取 PHNQs 的产率高于树脂。采用高效液相色谱（HPLC）与二极管阵列检测和质谱联用对 PHNQ 组成进行了表征。鉴定了五种 PHNQ 化合物（棘色素 E、B、C、A 和海胆素 A）和三种胺化 PHNQ 化合物（棘胺 E、海胆胺 A 和 B）。这些色素在暴露于光时容易降解，其中胺化 PHNQ 色素最不稳定。