从海胆（Evechinus chloroticus）中提取的 PHNQs 的体外抗氧化和抗菌活性以及体内抗炎活性的研究。

In vitro antioxidant and antimicrobial activities, and in vivo anti-inflammatory activity of crude and fractionated PHNQs from sea urchin (Evechinus chloroticus).

机构信息

Department of Food Science, University of Otago, Dunedin, New Zealand.

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

出版信息

Food Chem. 2020 Jun 30;316:126339. doi: 10.1016/j.foodchem.2020.126339. Epub 2020 Jan 31.

DOI:10.1016/j.foodchem.2020.126339

PMID:32044704

Abstract

This study investigated the bioactivities of polyhydroxyl-1,4-naphthoquinone (PHNQ) extracts from Evechinus chloroticus shell waste. PHNQs were extracted from E. chloroticus shells and spines using different solvents and the crude extracts were fractionated by HPLC. The antioxidant activity of the PHNQ extracts were evaluated by the DPPH, ABTS, ORAC and FRAP assays. Ethyl acetate was the best extraction solvent and spine extracts showed better antioxidant activity than shell extracts (p < 0.05). The HPLC fraction containing spinochrome E showed the highest antioxidant activity (p < 0.05). The Minimum Inhibitory Concentration of the PHNQ crude extracts and the HPLC fractions ranged from 250 to 2500 µg/mL depending on the PHNQ extract and microbial species tested. Treatment by PHNQ extracts resulted in alteration of the morphology of the microbial cell wall as observed by transmission electron microscopy. PHNQ extracts also exhibited anti-inflammatory activity in rats (ED = 8.26 ± 0.22 µg), comparable to that of Celecoxib (6.12 ± 0.18 µg).

摘要

本研究考察了从皱瘤海胆壳废物中提取的多羟基-1,4-萘醌（PHNQ）提取物的生物活性。使用不同溶剂从皱瘤海胆壳和刺中提取 PHNQ，并通过 HPLC 对粗提取物进行分级。通过 DPPH、ABTS、ORAC 和 FRAP 测定法评估 PHNQ 提取物的抗氧化活性。乙酸乙酯是最佳的提取溶剂，刺提取物的抗氧化活性优于壳提取物（p<0.05）。含有海胆色素 E 的 HPLC 馏分显示出最高的抗氧化活性（p<0.05）。根据所测试的 PHNQ 提取物和微生物种类，PHNQ 粗提取物和 HPLC 馏分的最小抑菌浓度范围为 250 至 2500μg/mL。PHNQ 提取物处理导致微生物细胞壁形态发生变化，通过透射电子显微镜观察到。PHNQ 提取物在大鼠中也表现出抗炎活性（ED=8.26±0.22μg），与塞来昔布（6.12±0.18μg）相当。