Zhang Yanrong, Liao Chengde, Qu Haibo, Huang Siqin, Jiang Hong, Zhou Haiyan, Abrams Emily, Habte Frezghi G, Yuan Li, Bertram Edward H, Lee Kevin S, Pauly Kim Butts, Buckmaster Paul S, Wintermark Max
Department of Ultrasound, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, China; Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA.
Neuroradiology Section, Department of Radiology, Stanford University, Stanford, California, USA; Department of Radiology, Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, Yunnan, China.
Ultrasound Med Biol. 2019 Jan;45(1):129-136. doi: 10.1016/j.ultrasmedbio.2018.08.023. Epub 2018 Oct 8.
The goal of this study was to test different combinations of acoustic pressure and doses of quinolinic acid (QA) for producing a focal neuronal lesion in the murine hippocampus without causing unwanted damage to adjacent brain structures. Sixty male CD-1 mice were divided into 12 groups that underwent magnetic resonance-guided focused ultrasound at high (0.67 MPa), medium (0.5 MPa) and low (0.33 MPa) acoustic peak negative pressures and received QA at high (0.012 mmol), medium (0.006 mmol) and low (0.003 mmol) dosages. Neuronal loss occurred only when magnetic resonance-guided focused ultrasound with adequate acoustic power (0.67 or 0.5 MPa) was combined with QA. The animals subjected to the highest acoustic power had larger lesions than those treated with medium acoustic power, but two mice had evidence of bleeding. When the intermediate acoustic power was used, medium and high dosages of QA produced lesions larger than those produced by the low dosage.
本研究的目的是测试声压与喹啉酸（QA）剂量的不同组合，以便在不造成相邻脑结构不必要损伤的情况下，在小鼠海马体中产生局灶性神经元损伤。60只雄性CD-1小鼠被分为12组，分别接受高（0.67兆帕）、中（0.5兆帕）和低（0.33兆帕）声峰负压的磁共振引导聚焦超声，并接受高（0.012毫摩尔）、中（0.006毫摩尔）和低（0.003毫摩尔）剂量的QA。仅当具有足够声功率（0.67或0.5兆帕）的磁共振引导聚焦超声与QA联合使用时，才会发生神经元丢失。接受最高声功率的动物比接受中等声功率治疗的动物有更大的损伤，但有两只小鼠有出血迹象。当使用中等声功率时，中高剂量的QA产生的损伤比低剂量产生的损伤更大。
