College of Animal Science, Inner Mongolia Agricultural University, Hohhot, PR China.
UWA Institute of Agriculture, The University of Western Australia, Crawley, WA, Australia.
J Anim Sci. 2019 Jan 1;97(1):385-397. doi: 10.1093/jas/sky386.
The aim of this study was to investigate the effects of dietary supplementation with heated linseed on the fatty acid (FA) composition of the plasma, liver, and subcutaneous adipose tissue (SADT) of Albas white cashmere kids, particularly the effect on n-3 long-chain polyunsaturated FA profiles and the mRNA expression of genes related to lipid metabolism in SADT. Sixty 4-month-old castrated male kids (average BW 18.6 ± 0.1 kg) were selected and randomly allocated into three groups in a randomized block design. Three dietary treatments were used: (1) basal diet without supplementation (Control), (2) basal diet supplemented with linseed oil (LSO), and (3) basal diet supplemented with heated linseed grain (HLS). The diets were fed for 104 d, consisting of 14 d for adaptation followed by 90 d of measurement. Different FA profiles were found in SADT between LSO and HLS. Kids fed HLS had more C18:3n3 (P < 0.0001), C22:6n3 (P = 0.007), and n-3 PUFA (P < 0.0001) and a less (P < 0.0001) n-6/n-3 ratio than LSO kids. These FA differences between LSO and HLS kids were due to the increased expression of elongation of very long chain FA protein 5 (P < 0.0001), delta-6 desaturase (P < 0.0001), and peroxisome proliferator-activated receptor α (P = 0.003) in SADT of HLS kids and was also associated with liver fat metabolism. Together, these results suggest that the consumption of HLS leads to more C22:6n3 than LSO in SADT by increasing liver C22:6n3 content and by increasing SADT mRNA expression of ELOVL5 and FADS2 through promoting PPARα expression.
本研究旨在探究饲粮添加加热亚麻籽对阿尔巴斯白绒山羊羔羊血浆、肝脏和皮下脂肪组织（SADT）脂肪酸（FA）组成的影响，特别是对 n-3 长链多不饱和 FA 谱和 SADT 中与脂代谢相关基因的 mRNA 表达的影响。选择 60 只 4 月龄去势雄性羔羊（平均 BW 18.6 ± 0.1 kg），采用完全随机分组设计，分为 3 个组，每个组 20 个重复，每个重复 1 只羊。3 种饲粮处理如下：（1）基础饲粮不添加（对照）；（2）基础饲粮添加亚麻籽油（LSO）；（3）基础饲粮添加加热亚麻籽（HLS）。试验期 104 d，预试期 14 d，正试期 90 d。LSO 和 HLS 组 SADT 中 FA 谱不同。与 LSO 组相比，HLS 组羔羊 C18:3n3（P < 0.0001）、C22:6n3（P = 0.007）和 n-3 PUFA 含量更高（P < 0.0001），n-6/n-3 比值更低（P < 0.0001）。HLS 组羔羊 SADT 中 ELOVL5、delta-6 去饱和酶和过氧化物酶体增殖物激活受体α的 mRNA 表达增加（P < 0.0001），导致 LSO 和 HLS 组羔羊 SADT 中 FA 差异，这与肝脏脂肪代谢有关。综上，饲粮添加 HLS 通过增加肝脏 C22:6n3 含量和促进 PPARα 表达，增加 SADT 中 ELOVL5 和 FADS2 的 mRNA 表达，使 HLS 组羔羊 SADT 中 C22:6n3 含量高于 LSO 组。
