促性腺激素释放激素激动剂可诱导子宫内膜异位症患者腱蛋白 1 的下调。

Gonadotropin-releasing hormone agonist induces downregulation of tensin 1 in women with endometriosis.

机构信息

Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

出版信息

Acta Obstet Gynecol Scand. 2019 Feb;98(2):222-231. doi: 10.1111/aogs.13481. Epub 2018 Nov 11.

DOI:10.1111/aogs.13481

PMID:30312486

Abstract

INTRODUCTION

Many cell migration-related molecules are associated with endometriosis. Tensin 1 (TNS1), which has been implicated in cell migration, may play a role in endometriosis. The study goal was to evaluate the TNS1 expression in endometrial tissue and serum from women with endometriosis treated with gonadotropin-releasing hormone agonist (GnRHa).

MATERIAL AND METHODS

Tissue and serum samples were collected from women with endometriosis who were treated (n = 29) with GnRHa or untreated (n = 30). TNS1 mRNA was examined using quantitative PCR. TNS1 protein levels in tissue and serum samples were investigated using Western blot, immunohistochemistry and ELISA. Eleven women with endometriosis participated in a follow-up investigation of serum TNS1 before and after GnRHa treatment.

RESULTS

TNS1 mRNA (P = 0.006) and protein (P = 0.001) were significantly downregulated in endometriotic tissue from women with endometriosis who received GnRHa. Immunolocalization of TNS1 showed strong expression in the epithelial and stromal cells of endometriotic tissue from women untreated with GnRHa, whereas endometriotic tissue from GnRHa-treated women showed low TNS1 expression. Follow-up monitoring of serum TNS1 concentration in 11 women showed an average decrease in concentration of 53%, from 294.9 ± 66.69 to 140.3 ± 55.21 pg/mL, following GnRHa treatment (P = 0.003).

CONCLUSIONS

GnRHa induces downregulation of TNS1 in tissue and serum in women with endometriosis. These results emphasize the importance TNS1 as a potential therapeutic molecular target for the treatment of endometriosis with GnRHa.

摘要

简介

许多与细胞迁移相关的分子与子宫内膜异位症有关。张力蛋白 1（TNS1）与细胞迁移有关，可能在子宫内膜异位症中发挥作用。本研究旨在评估接受促性腺激素释放激素激动剂（GnRHa）治疗的子宫内膜异位症患者的子宫内膜组织和血清中的 TNS1 表达。

材料和方法

收集接受 GnRHa 治疗（n=29）或未治疗（n=30）的子宫内膜异位症患者的组织和血清样本。使用定量 PCR 检测 TNS1 mRNA。使用 Western blot、免疫组织化学和 ELISA 检测组织和血清样本中的 TNS1 蛋白水平。11 名子宫内膜异位症患者参与了 GnRHa 治疗前后血清 TNS1 的随访研究。

结果

接受 GnRHa 治疗的子宫内膜异位症患者的子宫内膜组织中 TNS1 mRNA（P=0.006）和蛋白（P=0.001）表达显著下调。GnRHa 未治疗的子宫内膜异位症组织中 TNS1 的免疫定位显示上皮和基质细胞表达强烈，而 GnRHa 治疗的子宫内膜异位症组织中 TNS1 表达较低。对 11 名妇女的血清 TNS1 浓度进行随访监测显示，GnRHa 治疗后浓度平均下降 53%，从 294.9±66.69 pg/mL 降至 140.3±55.21 pg/mL（P=0.003）。