Yuan Zhiyong, Jin Jieqiong, Li Jiannan, Stuart Bryan L, Wu Jun
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,, Southwest Forestry University, Kunming 650224, Yunnan, China.
Zootaxa. 2018 May 1;4415(3):498-512. doi: 10.11646/zootaxa.4415.3.5.
A new species, Amolops wenshanensis sp. nov., is described from Guangxi and Yunnan Provinces, China. The new species is a member of the A. monticola group, and is distinguished from its congeners using morphological and molecular data. The new species is diagnosed by having glandular dorsolateral folds; smooth skin; side of head dark with a light-colored upper lip stripe extending to axilla; green dorsal coloration in life; immaculate venter; indistinct transverse bands on dorsal surfaces of limbs; tympanum distinct; pineal body absent; all fingertips expanded with circummarginal grooves; two oblique vomerine teeth; vocal sac and white nuptial pad present in males; supratympanic fold absent; outer metatarsal tubercle absent; glandular gold-white flank spot absent; and skin on venter not translucent. A molecular phylogenetic analysis is performed using the mitochondrial (mt) NADH dehydrogenase subunit 2 (ND2) gene and parts of flanking tRNA genes for nine of the 15 members of the A. monticola group with available homologous sequences. The new species is sister to a clade containing A. cucae and A. compotrix, two species known from adjacent parts of Vietnam and Laos. The new species differs from its congeners by having an uncorrected p-distance of > 6.7% in the mt DNA fragment examined. At present, the new species is known from small, montane streams near Wenshan, Yunnan Province, and Jingxi, Guangxi Province, China.
一种新物种——文山湍蛙（Amolops wenshanensis sp. nov.）在中国广西和云南省被描述。该新物种是山湍蛙组（A. monticola group）的成员之一，通过形态学和分子数据与同属其他物种相区分。该新物种的鉴别特征如下：具背外侧腺褶；皮肤光滑；头部侧面深色，浅色的上唇纹延伸至腋窝；生活时背部呈绿色；腹部无斑；四肢背面无明显横纹；鼓膜明显；无松果体；所有指尖膨大且具边缘沟；有两枚斜向的犁骨齿；雄性具声囊和白色婚垫；无颞褶；无外跖突；无金色的胁腺斑；腹部皮肤不透明。利用线粒体（mt）NADH脱氢酶亚基2（ND2）基因及侧翼部分tRNA基因，对山湍蛙组15个成员中9个具有可用同源序列的物种进行了分子系统发育分析。该新物种是包含越南和老挝相邻地区已知的库氏湍蛙（A. cucae）和康氏湍蛙（A. compotrix）的一个分支的姐妹种。在所检测的线粒体DNA片段中，该新物种与其同属其他物种的未校正p距离大于6.7%。目前，该新物种仅发现于中国云南省文山附近及广西壮族自治区靖西的小型山间溪流中。
