Watabe T, Tanaka K, Kumagae M, Itoh S, Hasegawa M, Horiuchi T, Miyabe S, Ohno H, Shimizu N
Life Sci. 1987 Apr 27;40(17):1651-5. doi: 10.1016/0024-3205(87)90013-0.
Plasma corticotropin-releasing factor (CRF), corticotropin (ACTH) and cortisol levels were simultaneously determined by radioimmunoassays at 0600 h, 1200 h, 1800 h and 2200 h in six normal subjects, in order to examine whether the diurnal rhythm in plasma CRF exists and how it correlates to the diurnal rhythm in plasma ACTH and cortisol concentration. The highest CRF level was observed at 0600 h (7.0 +/- 1.2 pg/ml) and significantly lower levels (p less than 0.01) at 1800 h (1.7 +/- 0.2 pg/ml) and 2200 h (1.9 +/- 0.4 pg/ml). A clear diurnal rhythm was demonstrated in plasma ACTH and cortisol levels, with the highest values at 0600 h (44.6 +/- 8.1 pg/ml and 15.9 +/- 2.0 micrograms/dl, respectively) and the lowest at 2200 h (12.3 +/- 2.8 pg/ml and 4.6 +/- 1.0 micrograms/ml, respectively). These results suggest that the diurnal rhythm in ACTH and cortisol is under the regulation, at least in part, of the diurnal rhythm in CRF secretion.
为了研究血浆促肾上腺皮质激素释放因子（CRF）是否存在昼夜节律以及它与血浆促肾上腺皮质激素（ACTH）和皮质醇浓度的昼夜节律如何相关，采用放射免疫分析法在06:00、12:00、18:00和22:00同时测定了6名正常受试者的血浆CRF、ACTH和皮质醇水平。CRF的最高水平出现在06:00（7.0±1.2 pg/ml），而在18:00（1.7±0.2 pg/ml）和22:00（1.9±0.4 pg/ml）时显著降低（p<0.01）。血浆ACTH和皮质醇水平呈现明显的昼夜节律，06:00时达到最高值（分别为44.6±8.1 pg/ml和15.9±2.0 μg/dl），22:00时降至最低（分别为12.3±2.8 pg/ml和4.6±1.0 μg/ml）。这些结果表明，ACTH和皮质醇的昼夜节律至少部分受CRF分泌昼夜节律的调节。
