Cazarotto Chrystian J, Boito Jhonatan P, Gebert Roger R, Reis João H, Machado Gustavo, Bottari Nathieli B, Morsch Vera M, Schetinger Maria R C, Doleski Pedro H, Leal Marta L R, Baldissera Matheus D, Da Silva Aleksandro S
Universidade do Estado de Santa Catarina (UDESC), Chapecó, Santa Catarina (SC), Brazil.
Veterinary Population Medicine, University of Minnesota, Sait Paul, MN, United States.
Res Vet Sci. 2018 Dec;121:46-52. doi: 10.1016/j.rvsc.2018.09.003. Epub 2018 Sep 28.
The aim of this study was to evaluate the metaphylactic effect of minerals on immunological and antioxidant responses, as well as performance and prevention of coccidiosis in newborn lambs. We divided 110 newborn lambs into two groups (55/group): control (untreated) and treated with two doses of 0.33 mL/kg of a mineral complex (zinc, copper, selenium, and manganese) on day of life (DOL) 1 and 30. Total blood was collected at DOL 1, 15, 30 and 45 to measure antioxidant enzymes, biochemical and immunology analyses, and haemogram. Treated animals were heavier (P < .05) than untreated lambs on DOL 15 and 45, but not on DOL 30 due to a coccidiosis outbreak. Catalase activity did not differ between groups, while superoxide dismutase and xanthine oxidase activities were higher (P < .05) in treated lambs compared with control animals. Serum levels of total protein and globulins were higher (P < .05) in treated animals (DOL 15, 30 and 45). A significant increased on the number of lymphocytes (DOL 45), as well as on seric levels of immunoglobulins (IgM and IgG) was observed in treated animals (DOL 15 and 30). Serum Ig levels remained constant throughout the experiment in the treated group, but fluctuated in the control group. Serum glucose levels were greater in treated animals (DOL 15 and 30). It is possible to conclude that subcutaneous administration of minerals has beneficial effects on lambs by increasing antioxidant and immunological defenses, reflected by greater weight gain, which could mitigate the impact of coccidiosis.
本研究的目的是评估矿物质对新生羔羊免疫和抗氧化反应的群体预防作用，以及对其生长性能和球虫病预防的影响。我们将110只新生羔羊分为两组（每组55只）：对照组（未处理）和处理组，处理组在出生第1天和第30天分别皮下注射两剂0.33 mL/kg的矿物质复合物（锌、铜、硒和锰）。在出生第1天、15天、30天和45天采集全血，以检测抗氧化酶、进行生化和免疫学分析以及血常规检查。在出生第15天和第45天，处理组羔羊体重比未处理组重（P<0.05），但在出生第30天，由于球虫病爆发，两组体重无差异。两组间过氧化氢酶活性无差异，而处理组羔羊超氧化物歧化酶和黄嘌呤氧化酶活性高于对照组（P<0.05）。处理组动物血清总蛋白和球蛋白水平较高（P<0.05）（出生第15天、30天和45天）。处理组动物淋巴细胞数量显著增加（出生第45天），血清免疫球蛋白（IgM和IgG）水平也显著增加（出生第15天和30天）。在整个实验过程中，处理组血清Ig水平保持恒定，而对照组则有波动。处理组动物血清葡萄糖水平较高（出生第15天和第30天）。可以得出结论，皮下注射矿物质对羔羊具有有益作用，可增强抗氧化和免疫防御，表现为体重增加更多，这可能减轻球虫病的影响。
