Zhang Limei
Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE, USA.
Methods Mol Biol. 2019;1876:179-195. doi: 10.1007/978-1-4939-8864-8_12.
X-ray absorption spectroscopy (XAS) has been widely used as a powerful local structural tool for studying the metal binding sites in metalloproteins, owing to its element specificity, chemical sensitivity, and minimum requirement for sample preparation. The principle subject of this chapter is to provide a general introduction of this technique to molecular biologists and biochemists, with the intention to fill the knowledge gaps in interdisciplinary communication and collaboration. An update of the XAS-based technique applied recently to metalloproteins is also briefly introduced at the end of the chapter.
X射线吸收光谱（XAS）作为一种强大的局部结构分析工具，已被广泛应用于研究金属蛋白中的金属结合位点，这得益于其元素特异性、化学敏感性以及对样品制备的最低要求。本章的主要目的是向分子生物学家和生物化学家全面介绍这项技术，以填补跨学科交流与合作中的知识空白。本章末尾还简要介绍了最近应用于金属蛋白的基于XAS的技术的最新进展。
