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着丝粒许可酵母染色体臂有丝分裂的浓缩。

Centromeres License the Mitotic Condensation of Yeast Chromosome Arms.

机构信息

Institute of Biochemistry, Biology Department, ETH Zurich, 8093 Zurich, Switzerland.

Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway.

出版信息

Cell. 2018 Oct 18;175(3):780-795.e15. doi: 10.1016/j.cell.2018.09.012. Epub 2018 Oct 11.

DOI:10.1016/j.cell.2018.09.012
PMID:30318142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6197839/
Abstract

During mitosis, chromatin condensation shapes chromosomes as separate, rigid, and compact sister chromatids to facilitate their segregation. Here, we show that, unlike wild-type yeast chromosomes, non-chromosomal DNA circles and chromosomes lacking a centromere fail to condense during mitosis. The centromere promotes chromosome condensation strictly in cis through recruiting the kinases Aurora B and Bub1, which trigger the autonomous condensation of the entire chromosome. Shugoshin and the deacetylase Hst2 facilitated spreading the condensation signal to the chromosome arms. Targeting Aurora B to DNA circles or centromere-ablated chromosomes or releasing Shugoshin from PP2A-dependent inhibition bypassed the centromere requirement for condensation and enhanced the mitotic stability of DNA circles. Our data indicate that yeast cells license the chromosome-autonomous condensation of their chromatin in a centromere-dependent manner, excluding from this process non-centromeric DNA and thereby inhibiting their propagation.

摘要

在有丝分裂过程中，染色质凝聚将染色体塑造成独立、刚性和紧凑的姐妹染色单体，以促进其分离。在这里，我们表明，与野生型酵母染色体不同，非染色体 DNA 环和没有着丝粒的染色体在有丝分裂过程中不会发生凝聚。着丝粒通过招募激酶 Aurora B 和 Bub1 在顺式严格促进染色体凝聚，这触发了整个染色体的自主凝聚。Shugoshin 和去乙酰化酶 Hst2 有助于将凝聚信号传播到染色体臂上。将 Aurora B 靶向 DNA 环或着丝粒缺失的染色体，或从 PP2A 依赖性抑制中释放 Shugoshin，可以绕过着丝粒对凝聚的要求，并增强 DNA 环的有丝分裂稳定性。我们的数据表明，酵母细胞以依赖着丝粒的方式许可其染色质的染色体自主凝聚，从而排除非着丝粒 DNA 参与该过程，从而抑制其复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/cf76b9dc1cdd/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/07115a0595be/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/8e5c9124e5bc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/7b462bd33566/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/7d12597e43b6/figs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/d62231214698/figs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/0332e091a893/figs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/0a3502772dfb/figs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/7b876a7996d1/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/806ed93c5d2b/figs6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/87bd5a912e1f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/f6d2a7d79bdd/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/a565b8612e97/figs7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/a2ab6fdc5363/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/6f39dd965a47/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/cf76b9dc1cdd/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/07115a0595be/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/8e5c9124e5bc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/7b462bd33566/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/7d12597e43b6/figs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/d62231214698/figs3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/0332e091a893/figs4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/0a3502772dfb/figs5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/7b876a7996d1/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/806ed93c5d2b/figs6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/87bd5a912e1f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/f6d2a7d79bdd/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/a565b8612e97/figs7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/a2ab6fdc5363/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/6f39dd965a47/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/069e/6197839/cf76b9dc1cdd/gr7.jpg

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