采用高效液相色谱法结合多变量分析对希托帕拉迪 churn 进行质量评估。

Quality Assessment of Sitopaladi Churna Using High-Performance Liquid Chromatography Coupled with Multivariate Analysis.

作者信息

Patel Nikunj D, Prajapati Prajesh N, Kanaki Niranjan S

机构信息

Department of Pharmaceutical sciences, K.B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Sarva Vidyalaya Campus, GH-6 Circle, Sector 23, Gandhinagar 382023, India.

Institute of Research and Development, Gujarat Forensic Sciences University, National Highway 8C, near DFS Head Quarters, Sector 9, Gandhinagar 382007, India.

出版信息

J Chromatogr Sci. 2020 Oct 26;58(10):961-968. doi: 10.1093/chromsci/bmaa070.

DOI:10.1093/chromsci/bmaa070

PMID:33015709

Abstract

"Sitopaladi churna," a well-known formulation of Ayurveda, is prescribed to treat the disease like Bronchitis, Pneumonia, allergic conditions, viral infection of the respiratory tract and as a natural antioxidant. A novel method based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector was established and validated for sitopaladi churna. Here, HPLC fingerprints data obtained for 28 samples including standard sample were then treated to chemometric analysis like principal component analysis and hierarchical clustering analysis for further analysis to evaluate the differences in market samples of sitopaladi churna. Additionally, one major marker compound, piperine was quantified and it also facilitated for relative retention time. The simulative mean spectrum was also generated. The validation results showed that the developed method was simple, precise and stable. Thus, the developed chromatographic method adjoined with multivariate analysis can be used as an efficient and practical approach for quality assessment of sitopaladi churna.

摘要

“希托帕拉迪 churnam”是阿育吠陀中一种著名的配方，被用于治疗如支气管炎、肺炎、过敏病症、呼吸道病毒感染等疾病，还可作为一种天然抗氧化剂。建立了一种基于反相高效液相色谱（RP-HPLC）与光电二极管阵列检测器联用的新方法，并对希托帕拉迪 churnam进行了验证。在此，对包括标准样品在内的28个样品获得的HPLC指纹图谱数据进行主成分分析和层次聚类分析等化学计量分析，以进一步评估希托帕拉迪 churnam市售样品的差异。此外，对一种主要标记化合物胡椒碱进行了定量分析，这也有助于确定相对保留时间。还生成了模拟平均光谱。验证结果表明，所开发的方法简便、精确且稳定。因此，所开发的色谱方法与多变量分析相结合，可作为希托帕拉迪 churnam质量评估的一种高效实用方法。