Mayr Vanessa, Sturtzel Caterina, Stadler Manuela, Grissenberger Sarah, Distel Martin
Innovative Cancer Models, St. Anna Kinderkrebsforschung, Children's Cancer Research Institute, Vienna, Austria.
Front Cell Dev Biol. 2018 Sep 25;6:111. doi: 10.3389/fcell.2018.00111. eCollection 2018.
Precise regulation of signaling pathways in single cells underlies tissue development, maintenance and repair in multicellular organisms, but our ability to monitor signaling dynamics in living vertebrates is currently limited. We implemented kinase translocation reporter (KTR) technology to create DREKA ("ynamic eporter of rctivity") zebrafish, which allow one to observe Erk activity at single cell level with high temporal resolution. DREKA zebrafish faithfully reported Erk activity after muscle cell wounding and revealed the kinetics of small compound uptake. Our results promise that kinase translocation reporters can be adapted for further applications in developmental biology, disease modeling, and pharmacology in zebrafish.
单细胞中信号通路的精确调控是多细胞生物组织发育、维持和修复的基础，但目前我们监测活体脊椎动物信号动态的能力有限。我们应用激酶易位报告基因（KTR）技术创建了DREKA（“活性动态报告基因”）斑马鱼，它能让人们在单细胞水平以高时间分辨率观察Erk活性。DREKA斑马鱼如实地报告了肌肉细胞受伤后的Erk活性，并揭示了小分子化合物摄取的动力学。我们的结果表明，激酶易位报告基因可适用于斑马鱼发育生物学、疾病建模和药理学的进一步应用。
