Waldbauer Katharina, Seiringer Günter, Sykora Christina, Dirsch Verena M, Zehl Martin, Kopp Brigitte
Faculty of Life Sciences, Department of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.
Faculty of Chemistry, Department of Analytical Chemistry, University of Vienna, Währingerstrasse 38, 1090 Vienna, Austria.
ACS Omega. 2018 Sep 30;3(9):10545-10553. doi: 10.1021/acsomega.8b00638. Epub 2018 Sep 5.
Pomace, the press residue from different fruits accumulating as waste product in food industry, contains high amounts of secondary metabolites that could be utilized for health-related applications. This study aims at evaluating the potential of pomaces of apricot, bilberry, and elderberry to serve as a source for endothelial nitric oxide synthase (eNOS)-activating compounds. Five extracts obtained from the lyophilized pomace of apricot and elderberry with solvents of different polarity were found to enhance A23187-stimulated eNOS activity when tested at 50 μg/mL in an [C]-l-arginine to [C]-l-citrulline conversion assay in the human endothelium-derived cell line EA.hy926 ( < 0.05). The bioassay-guided fractionation of the extracts obtained with methanol/water (70:30) led to several active fractions from apricot pomace ( < 0.05) and elderberry pomace ( < 0.01). Liquid chromatography-mass spectrometry-based chemical analysis of the extracts and active fractions pointed mainly to triterpenoic acids as active compounds. One particular dihydroxytriterpenoic acid, characteristic for elderberry, was enriched as the main compound in the two most active fractions and might serve as a promising lead structure for further studies.
果渣是食品工业中作为废弃物积累的不同水果的压榨残渣，含有大量可用于健康相关应用的次生代谢产物。本研究旨在评估杏、越橘和接骨木果渣作为内皮型一氧化氮合酶（eNOS）激活化合物来源的潜力。在人内皮细胞系EA.hy926的[C]-L-精氨酸到[C]-L-瓜氨酸转化试验中，当以50μg/mL进行测试时，发现从杏和接骨木果的冻干果渣中用不同极性溶剂获得的五种提取物可增强A23187刺激的eNOS活性（<0.05）。对用甲醇/水（70:30）获得的提取物进行生物测定导向的分级分离，得到了来自杏果渣（<0.05）和接骨木果渣（<0.01）的几个活性级分。基于液相色谱-质谱的提取物和活性级分化学分析主要表明三萜酸是活性化合物。一种特定的二羟基三萜酸是接骨木果的特征性成分，在两个活性最高的级分中作为主要化合物富集，可能作为进一步研究的有前景的先导结构。
