Gupta Mamta, Zha Jian, Zhang Xing, Jung Gyoo Yeol, Linhardt Robert J, Koffas Mattheos A G
Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States.
Department of Botany and Environment Studies, DAV University, Jalandhar 144 001, Punjab, India.
ACS Omega. 2018 Sep 30;3(9):11643-11648. doi: 10.1021/acsomega.8b01134. Epub 2018 Sep 24.
Anthocyanins are plant secondary metabolites that, despite their chemical instability, have found many applications as natural food colorants. They are also known for their beneficial health effects because of their antioxidant and anticancer properties. More stable versions of these molecules, particularly at neutral pH conditions, are required to study the anthocyanin pharmacokinetic properties and obtain effective therapeutic results. In the present report, a cost-effective technique was developed to prepare the deuterated anthocyanin using recombinant as a production host and deuterated glycerol and DO in the culture media. This approach resulted in the formation of endogenous deuterated uridine 5'-diphosphate-glucose that was further incorporated by the recombinant anthocyanin pathway, resulting in the formation of deuterated cyanidin 3--glucoside (C3G). The deuterium exchange of O-D and C-D were studied by liquid chromatography (LC)-mass spectrometry and NMR analysis. The labeled C3G, purified by high-performance LC showed a stable nature at pH 7.0 as compared to nondeuterated C3G.
花青素是植物次生代谢产物，尽管其化学性质不稳定，但已作为天然食用色素得到了广泛应用。它们还因其抗氧化和抗癌特性而具有有益的健康效应。为了研究花青素的药代动力学特性并获得有效的治疗效果，需要这些分子更稳定的形式，特别是在中性pH条件下。在本报告中，开发了一种经济高效的技术，以重组体作为生产宿主，并在培养基中使用氘代甘油和重水来制备氘代花青素。这种方法导致形成内源性氘代尿苷5'-二磷酸葡萄糖，其进一步通过重组花青素途径掺入，从而形成氘代矢车菊素3-葡萄糖苷（C3G）。通过液相色谱（LC）-质谱和NMR分析研究了O-D和C-D的氘交换。通过高效液相色谱纯化的标记C3G与未氘代的C3G相比，在pH 7.0时表现出稳定的性质。
