Volm M, Mattern J, Vogt-Schaden M, Wayss K
Anticancer Res. 1987 Jan-Feb;7(1):71-6.
Specimens of primary lung carcinomas and lymph node metastases from the same 18 patients were investigated by means of flow cytometry. The number of DNA stemlines, DNA indices, the proportion of diploid cells in the tumors and the distribution of the cell cycle phases were compared. In 10 patients DNA stemlines and DNA indices were identical in primary tumors and metastases. In two cases the DNA indices were doubled in metastases. In 6 cases the primary tumors contained two abnormal DNA stemlines and their metastases contained only one aneuploid stemline. Gross differences between primary tumors and lymph node metastases with regard to the proportion of cell cycle phases could not be found. The large variation between primary tumors and lymph node metastases with regard to DNA stemlines indicates that flow cytometric analysis of lymph nodes gives only limited information about the primary tumors.
对来自18例患者的原发性肺癌及淋巴结转移灶标本进行了流式细胞术研究。比较了DNA干系数量、DNA指数、肿瘤中二倍体细胞的比例以及细胞周期各时相的分布。10例患者的原发性肿瘤和转移灶中DNA干系及DNA指数相同。2例转移灶中DNA指数翻倍。6例原发性肿瘤含有两条异常DNA干系，而其转移灶仅含有一条非整倍体干系。未发现原发性肿瘤与淋巴结转移灶在细胞周期各时相比例方面存在显著差异。原发性肿瘤与淋巴结转移灶在DNA干系方面的巨大差异表明，对淋巴结进行流式细胞术分析只能提供关于原发性肿瘤的有限信息。
