Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao, China.
Department of Patients Service Center, Qingdao Municipal Hospital, Qingdao 266000, China.
J Cell Biochem. 2019 Apr;120(4):5055-5061. doi: 10.1002/jcb.27782. Epub 2018 Oct 15.
Cyclic long noncoding RNAs have recently become major players in cancer biology and can serve as biomarkers for cancer diagnosis and prognosis, and as potential therapeutic targets. We explored circulating LINC00342 as a predictor of non-small cell lung cancer (NSCLC). The expression of LINC00342 in tissues, serum, PBMC, and NSCLC cell lines were screened by reverse transcription quantitative polymerase chain reaction. A multistage validation and risk score formula detection analysis was used. The effect of LINC00342 on proliferation was assessed by MTT, p53, and PTEN pathways, which were analyzed by Western blot analysis. We found that LINC00342 was upregulated in the tissues, serum, and PBMC of patients with NSCLC. In addition, patients with higher LINC00342 expression levels were associated with poor overall survival. For the diagnosis of NSCLC, the specificity and sensitivity of LINC00342 were significantly higher than that of CYFRA 21-1. Moreover, LINC00342 promoted proliferation by inhibiting the expression of p53 and PTEN proteins in NSCLC cell lines. Our study demonstrates that LINC00342 is involved in the development, and LINC00342 may be a potential diagnostic factor and a target for new therapies for future patients with NSCLC.
环状长非编码 RNA 最近已成为癌症生物学的主要参与者，可作为癌症诊断和预后的生物标志物，以及潜在的治疗靶点。我们探索了循环 LINC00342 作为非小细胞肺癌（NSCLC）的预测因子。通过逆转录定量聚合酶链反应筛选组织、血清、PBMC 和 NSCLC 细胞系中的 LINC00342 表达。采用多阶段验证和风险评分公式检测分析。通过 MTT、p53 和 PTEN 通路分析评估 LINC00342 对增殖的影响，通过 Western blot 分析进行分析。我们发现 LINC00342 在 NSCLC 患者的组织、血清和 PBMC 中上调。此外，LINC00342 表达水平较高的患者总生存期较差。对于 NSCLC 的诊断，LINC00342 的特异性和灵敏度明显高于 CYFRA 21-1。此外，LINC00342 通过抑制 NSCLC 细胞系中 p53 和 PTEN 蛋白的表达促进增殖。我们的研究表明，LINC00342 参与了 NSCLC 的发生发展，LINC00342 可能是未来 NSCLC 患者潜在的诊断因素和新治疗靶点。
