Halle W, Melzig M, Schössler W, Siems W E, Weidlich V, Teuscher E
Biomed Biochim Acta. 1986;45(10):1315-24.
The cell line BSEz-3 was established in a serum-reduced medium MEMPAS. The cells were isolated and cultivated under the same conditions as the calf aortic endothelial cell line BKEz-7. With regard to cell isolation and cell density in the stationary phase, BSEz-3-cells show characteristic differences from BKEz-7-cells. For the isolation of BSEz-3-cells an enzymatic-mechanical method was more successful than a mechanical technique only. The low saturation density in the stationary phase with an average number of 50,000 cells/cm2 of glass surface area is considered as a strong contact inhibition of cell proliferation. As a cell type specific marker the BSEz-3-cells contain factor-VIII-antigen and possess angiotensin-converting enzyme activity. For a practical use of the BSEz-3-cells in drug research we give recommandations regarding cell inoculum density for subcultivation and cryo-conservation in ampoules.
细胞系BSEz - 3是在低血清培养基MEMPAS中建立的。这些细胞与小牛主动脉内皮细胞系BKEz - 7在相同条件下分离和培养。关于细胞分离和稳定期的细胞密度，BSEz - 3细胞与BKEz - 7细胞表现出特征性差异。对于BSEz - 3细胞的分离，酶解 - 机械方法比仅用机械技术更成功。稳定期的低饱和密度，玻璃表面积平均每平方厘米50000个细胞，被认为是细胞增殖的强接触抑制。作为细胞类型特异性标志物，BSEz - 3细胞含有因子VIII抗原并具有血管紧张素转换酶活性。为了在药物研究中实际应用BSEz - 3细胞，我们给出了关于传代培养和安瓿冷冻保存的细胞接种密度的建议。
