Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, Minsk, Belarus.
Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, Minsk, Belarus.
Colloids Surf B Biointerfaces. 2019 Jan 1;173:412-420. doi: 10.1016/j.colsurfb.2018.10.009. Epub 2018 Oct 5.
Layer-by-layer (LbL) films with enhanced resistance to protein adsorption were obtained on the basis of N-grafted copolymers of chitosan with polyethylene glycol (PEG) or dextran (DEX). The copolymers with the backbone molecular weight of 18 and 450 kDa, side chains of PEG of 5.0 and 0.9 kDa, DEX of 6.0 kDa and the degree of amine groups substitution χ as high as ∼0.25 were alternated with dextran sulfate (DS) to assemble up to 10 bilayer films. The film material contains 85±5% of water with virtually no effect of the copolymer structure. By utilizing the graft copolymers and applying suitable number of copolymer/DS bilayers to the surface, the mass of adsorbed fetal bovine serum proteins was decreased by 70-85% as compared to that on unmodified chitosan/DS film. In terms of overlapping side chains on the LbL surface the copolymers of PEG and DEX are equally effective in tailoring protein-resistant materials.
基于接枝了聚乙二醇（PEG）或葡聚糖（DEX）的壳聚糖的 N-接枝共聚物，获得了具有增强的抗蛋白质吸附能力的层层（LbL）薄膜。共聚物的主链分子量为 18 和 450 kDa，PEG 的侧链分子量为 5.0 和 0.9 kDa，DEX 的侧链分子量为 6.0 kDa，胺基取代度 χ 高达 ∼0.25，与硫酸葡聚糖（DS）交替组装成多达 10 层薄膜。薄膜材料含有 85±5%的水，几乎不受共聚物结构的影响。通过利用接枝共聚物并在表面施加适当数量的共聚物/DS 双层，与未经修饰的壳聚糖/DS 薄膜相比，吸附胎牛血清蛋白的质量减少了 70-85%。就 LbL 表面上重叠的侧链而言，PEG 和 DEX 的共聚物在定制抗蛋白质材料方面同样有效。
Zotero 插件