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使用血红素、DNA和葡萄糖氧化酶分解葡萄糖敏感层层膜

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase.

作者信息

Yoshida Kentaro, Kashimura Yu, Kamijo Toshio, Ono Tetsuya, Dairaku Takenori, Sato Takaya, Kashiwagi Yoshitomo, Sato Katsuhiko

机构信息

School of Pharmaceutical Sciences, Ohu University 31-1 Misumido, Tomita-machi, Koriyama, Fukushima 963-8611, Japan.

Department of Creative Engineering, National Institute of Technology, Tsuruoka College, 104 Sawada, Inooka, Tsuruoka 997-8511, Japan.

出版信息

Polymers (Basel). 2020 Feb 4;12(2):319. doi: 10.3390/polym12020319.

DOI:10.3390/polym12020319
PMID:32033050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7077378/
Abstract

Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx) film was then partially decomposed by hydrogen peroxide (HO). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and HO, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx) film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx) films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1-10 mM.

摘要

通过层层（LbL）沉积血红素修饰的聚乙烯亚胺（H-PEI）溶液和DNA溶液（含有葡萄糖氧化酶（GOx））制备了葡萄糖敏感膜。利用静电相互作用构建了H-PEI/DNA + GOx多层膜。然后用过氧化氢（HO）使（H-PEI/DNA + GOx）膜部分分解。认为LbL膜分解的机制涉及血红素与HO反应形成的更多活性氧（ROS），进而导致非特异性DNA裂解。此外，LbL膜中存在的GOx与葡萄糖反应生成过氧化氢。因此，当薄膜浸入葡萄糖溶液中时，观察到（H-PEI/DNA + GOx）膜的分解。暴露于葡萄糖溶液24、48、72和96小时的（H-PEI/DNA + GOx）膜表明，膜的分解随时间增加，分别达到9.97%、16.3%、23.1%和30.5%。LbL膜的分解速率随葡萄糖浓度增加。在接近生理条件的pH和离子强度下，在1-10 mM的低葡萄糖浓度下可以缓慢分解LbL膜。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/5aa7c3813001/polymers-12-00319-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/d95144812bf4/polymers-12-00319-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/dae5effc45bf/polymers-12-00319-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/623f4ea62324/polymers-12-00319-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/c9ca2b2e144c/polymers-12-00319-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/121ccf51ba63/polymers-12-00319-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/2783db95d623/polymers-12-00319-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/ba960472c56f/polymers-12-00319-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/5aa7c3813001/polymers-12-00319-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/d95144812bf4/polymers-12-00319-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/dae5effc45bf/polymers-12-00319-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/623f4ea62324/polymers-12-00319-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/c9ca2b2e144c/polymers-12-00319-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/121ccf51ba63/polymers-12-00319-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/2783db95d623/polymers-12-00319-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/ba960472c56f/polymers-12-00319-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1d6/7077378/5aa7c3813001/polymers-12-00319-g008.jpg

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