Sadava D, Moore K
Biochem Biophys Res Commun. 1987 Mar 30;143(3):977-83. doi: 10.1016/0006-291x(87)90347-0.
Glycerol kinase activity was identified in extracts of higher plant seeds and seedlings, and was partially purified and characterized from cucumber radicle tissue. The enzyme was localized in the post-mitochondrial supernatant of the cell, and catalyzed the formation of glycerol-3-phosphate. The pH optiumum was 9.0. ATP, CTP, GTP or UTP could be used as the phosphoryl group donor. The Km for glycerol was 55 microM and Km values for the nucleoside triphosphates were 145-620 microM. The Vmax for the reaction was 40-78 pmol product per min. Kinetic data indicate that the enzyme has a sequential mechanism.
在高等植物种子和幼苗的提取物中鉴定出了甘油激酶活性，并从黄瓜胚根组织中对其进行了部分纯化和特性分析。该酶定位于细胞的线粒体后上清液中，催化甘油 -3- 磷酸的形成。最适pH为9.0。ATP、CTP、GTP或UTP均可作为磷酰基供体。甘油的Km值为55微摩尔，核苷三磷酸的Km值为145 - 620微摩尔。该反应的Vmax为每分钟40 - 78皮摩尔产物。动力学数据表明该酶具有有序机制。
