Kucharz E J
Connect Tissue Res. 1987;16(2):143-51. doi: 10.3109/03008208709002002.
Liver fibrosis in rats was induced by repeated subcutaneous injections of carbon tetrachloride. Total collagen, soluble and insoluble collagen fractions as well as type I and type III collagen content in the liver were subsequently measured over a 3-18 week period. Liver collagen was found to increase exponentially during this time. Insoluble collagen accumulated more rapidly than soluble forms, and the accumulation of type III collagen was relatively greater than type I collagen. Changes in specific liver enzymes were also observed. Collagenase, collagenolytic cathepsin and collagen peptidase activities all increased. Levels of collagen-degrading enzymes increased rapidly during the first weeks of fibrosis-induction, and were followed by a more gradual increase during the remainder of the study.
通过反复皮下注射四氯化碳诱导大鼠肝纤维化。随后在3至18周的时间内测量肝脏中的总胶原蛋白、可溶性和不溶性胶原蛋白组分以及I型和III型胶原蛋白含量。在此期间发现肝脏胶原蛋白呈指数增加。不溶性胶原蛋白比可溶性形式积累得更快，并且III型胶原蛋白的积累相对大于I型胶原蛋白。还观察到特定肝酶的变化。胶原酶、胶原分解组织蛋白酶和胶原肽酶活性均增加。在纤维化诱导的最初几周内，胶原蛋白降解酶的水平迅速增加，随后在研究的其余时间内逐渐增加。
