Perez-Tamayo R, Montfort I, Gonzalez E
Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City.
Exp Mol Pathol. 1987 Dec;47(3):300-8. doi: 10.1016/0014-4800(87)90015-3.
Collagenolytic activity has been measured in insoluble sediments of normal and CCl4-induced cirrhotic rat liver. Two techniques were used: in one assay, the insoluble liver sediments were incubated with a radioactively labeled exogenous substrate; in the other assay, the endogenous collagen present in the insoluble liver sediments served as the substrate. Animals in various stages of development of CCl4-induced cirrhosis were used. Results suggest that in rat liver cirrhosis collagenolytic activity assayed with both techniques appears to increase when expressed as weight of collagen degraded in 24 hr. On the other hand, when the data are corrected for the variable amounts of collagen present in the insoluble liver sediments and expressed as weight of collagen degraded in 24 hr per unit weight of total collagen incubated, collagenolytic activity is shown to be significantly decreased below the normal level and to remain so for prolonged periods.
已对正常大鼠和四氯化碳诱导的肝硬化大鼠肝脏的不溶性沉淀物中的胶原酶活性进行了测定。使用了两种技术：在一种测定方法中，将不溶性肝脏沉淀物与放射性标记的外源底物一起孵育；在另一种测定方法中，不溶性肝脏沉淀物中存在的内源性胶原作为底物。使用处于四氯化碳诱导的肝硬化不同发展阶段的动物。结果表明，在大鼠肝硬化中，用这两种技术测定的胶原酶活性，以24小时内降解的胶原重量表示时似乎会增加。另一方面，当对不溶性肝脏沉淀物中存在的可变胶原量进行校正，并以每单位孵育的总胶原重量在24小时内降解的胶原重量表示数据时，胶原酶活性显示显著低于正常水平，并在较长时间内保持如此。
