1 Department of Experimental Medicine, School of Medicine, University of Campania "Luigi Vanvitelli ," Naples, Italy .
2 Center of Biotechnologies , Antonio Cardarelli Hospital, Naples, Italy .
J Ocul Pharmacol Ther. 2018 Dec;34(10):677-684. doi: 10.1089/jop.2018.0046. Epub 2018 Oct 16.
The purpose of this in vitro study was to assess the potential benefits of eye drops based on hybrid cooperative complexes (HCCs) obtained from high and low molecular weight hyaluronic acid (HA).
Rheological measurements were performed to adjust the HCC concentration toward optimal resistance to drainage from the ocular surface. The viscosity and mucoadhesion profiles of the optimized preparation were derived. Primary porcine corneal epithelial cells were used for biological studies. Cells were exposed to dehydration after being pretreated with the HCC solution, and protection from desiccation was evaluated using cell viability assays. Time-lapse experiments were carried out to evaluate the ability of the HCC preparation to promote corneal wound healing. The characterization studies were performed in comparison with a control HA solution representative of commercial HA-based products.
The HCC formulation is able to deliver twice the amount of biopolymer compared with conventional products while avoiding discomfort due to excessive viscosity. The viscosity and mucoadhesion profiles allowed the authors to predict the longer in vivo retention and, therefore, an improved HCC formulation bioavailability. The new preparation also proved superior in protecting porcine corneal epithelial cells from desiccation and in hastening corneal cell wound repair in vitro.
The results suggest that the developed formulation may be a promising topical ophthalmic medical treatment.
本体外研究旨在评估基于高分子量和低分子量透明质酸（HA）的混合协同配合物（HCC）的滴眼剂的潜在益处。
进行流变学测量以调整 HCC 浓度，使其达到最佳抵抗眼表面排水的效果。得出优化制剂的粘度和黏膜黏附特性曲线。使用原代猪角膜上皮细胞进行生物学研究。将细胞用 HCC 溶液预处理后进行脱水处理，并通过细胞活力测定评估其对干燥的保护作用。进行时程实验以评估 HCC 制剂促进角膜伤口愈合的能力。与代表商业化基于 HA 的产品的对照 HA 溶液进行比较，进行了表征研究。
与传统产品相比，HCC 配方能够输送两倍量的生物聚合物，同时避免因过度粘度引起的不适。粘度和黏膜黏附特性曲线使作者能够预测更长的体内保留时间，从而提高 HCC 制剂的生物利用度。新制剂在保护猪角膜上皮细胞免受干燥和加速体外角膜细胞伤口修复方面也表现出优越性。
研究结果表明，所开发的制剂可能是一种有前途的局部眼科治疗药物。
