Precht Christina, Diserens Gaëlle, Vermathen Martina, Oevermann Anna, Lauper Josiane, Vermathen Peter
Clinical Radiology, Department of Clinical Veterinary Medicine, University of Bern, Bern, Switzerland.
AMSM, Departments of Radiology and Biomedical Research, University of Bern, Bern, Switzerland.
NMR Biomed. 2018 Dec;31(12):e4023. doi: 10.1002/nbm.4023. Epub 2018 Oct 17.
Listeria rhombencephalitis is caused by infection with Listeria monocytogenes and is associated with a high mortality rate in humans and ruminants. Little is known about the metabolic changes associated with neurolisteriosis in particular and infectious central nervous system (CNS) diseases in general. The purpose of our study was to investigate the metabolic changes associated with listeria rhombencephalitis in small ruminants (goats and sheep) as a model for inflammatory CNS disease by H high-resolution magic angle spinning nuclear magnetic resonance ( H HR-MAS NMR) spectroscopy of brain biopsies obtained from the brainstem and thalamus. Statistical analysis revealed distinct differences in the metabolic profile of brainstem biopsies, the primary location of listeria rhombencephalitis with moderate or severe inflammatory changes. N-Acetylaspartate (NAA), N-acetylaspartylglutamate, choline, myo-inositol and scyllo-inositol were decreased, and glycine, phosphocholine, taurine and lactate were increased, in the diseased group (n = 13) in comparison with the control group (n = 12). In the thalamus, which showed no or only mild inflammatory changes in the majority of animals, no statistically significant metabolic changes were observed. However, trends for metabolic alterations were partly the same as those found in the brainstem, including NAA, choline and lactate. This may be an indicator of metabolic changes occurring in the early stages of the disease. Therefore, further research with a larger number of animals is needed to evaluate the presence of subtle metabolic changes associated with mild inflammatory changes in the thalamus. In conclusion, H HR-MAS NMR investigation of listeria rhombencephalitis identified brain metabolite changes, offering new insights into the disease pathophysiology.
菱形脑炎由单核细胞增生李斯特菌感染引起，在人类和反刍动物中死亡率很高。人们对与神经李斯特菌病尤其是传染性中枢神经系统（CNS）疾病相关的代谢变化知之甚少。我们研究的目的是通过对取自脑干和丘脑的脑活检组织进行高分辨率魔角旋转核磁共振（1H HR-MAS NMR）光谱分析，以小型反刍动物（山羊和绵羊）为炎症性中枢神经系统疾病模型，研究与菱形脑炎相关的代谢变化。统计分析显示，脑干活检组织（菱形脑炎的主要发病部位，有中度或重度炎症变化）的代谢谱存在明显差异。与对照组（n = 12）相比，患病组（n = 13）的N-乙酰天门冬氨酸（NAA）、N-乙酰天门冬氨酰谷氨酸、胆碱、肌醇和 scyllo-肌醇减少，而甘氨酸、磷酸胆碱、牛磺酸和乳酸增加。在大多数动物中无或仅有轻度炎症变化的丘脑中，未观察到统计学上显著的代谢变化。然而，代谢改变的趋势部分与脑干中发现的相同，包括NAA、胆碱和乳酸。这可能是疾病早期发生代谢变化的一个指标。因此，需要对更多动物进行进一步研究，以评估丘脑中与轻度炎症变化相关的细微代谢变化的存在情况。总之，对菱形脑炎进行1H HR-MAS NMR研究确定了脑代谢物的变化，为该疾病的病理生理学提供了新的见解。
