Häni Annakatrin, Diserens Gaëlle, Oevermann Anna, Vermathen Peter, Precht Christina
Clinical Radiology, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Länggassstr. 124, 3012 Bern, Switzerland.
Departments of BioMedical Research and Radiology, University and Inselspital Bern, sitem-insel AG, Freiburgstr. 3, 3010 Bern, Switzerland.
Metabolites. 2021 Jan 6;11(1):38. doi: 10.3390/metabo11010038.
The metabolic profiling of tissue biopsies using high-resolution-magic angle spinning (HR-MAS) H nuclear magnetic resonance (NMR) spectroscopy may be influenced by experimental factors such as the sampling method. Therefore, we compared the effects of two different sampling methods on the metabolome of brain tissue obtained from the brainstem and thalamus of healthy goats by H HR-MAS NMR spectroscopy-in vivo-harvested biopsy by a minimally invasive stereotactic approach compared with postmortem-harvested sample by dissection with a scalpel. Lactate and creatine were elevated, and choline-containing compounds were altered in the postmortem compared to the in vivo-harvested samples, demonstrating rapid changes most likely due to sample ischemia. In addition, in the brainstem samples acetate and inositols, and in the thalamus samples ƴ-aminobutyric acid, were relatively increased postmortem, demonstrating regional differences in tissue degradation. In conclusion, in vivo-harvested brain biopsies show different metabolic alterations compared to postmortem-harvested samples, reflecting less tissue degradation. Sampling method and brain region should be taken into account in the analysis of metabolic profiles. To be as close as possible to the actual situation in the living individual, it is desirable to use brain samples obtained by stereotactic biopsy whenever possible.
使用高分辨率魔角旋转（HR-MAS）氢核磁共振（NMR）光谱对组织活检样本进行代谢谱分析可能会受到诸如采样方法等实验因素的影响。因此，我们通过HR-MAS NMR光谱比较了两种不同采样方法对从健康山羊脑干和丘脑获取的脑组织代谢组的影响——通过微创立体定向方法进行体内采集活检，与用手术刀解剖进行死后采集样本进行比较。与体内采集的样本相比，死后样本中的乳酸和肌酸升高，含胆碱化合物发生改变，这表明很可能由于样本缺血导致了快速变化。此外，在脑干样本中，死后乙酸盐和肌醇相对增加，在丘脑样本中，死后γ-氨基丁酸相对增加，这表明组织降解存在区域差异。总之，与死后采集的样本相比，体内采集的脑活检样本显示出不同的代谢改变，反映出组织降解较少。在分析代谢谱时应考虑采样方法和脑区。为了尽可能接近活体个体的实际情况，尽可能使用通过立体定向活检获得的脑样本。
