Šedo Ondrej, Radolfová-Křížová Lenka, Nemec Alexandr, Zdráhal Zbyněk
Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 10042 Prague, Czech Republic.
J Microbiol Methods. 2018 Nov;154:79-85. doi: 10.1016/j.mimet.2018.10.009. Epub 2018 Oct 15.
A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii. A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic clade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic clade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.
一组204株分类明确的菌株，属于17个不动杆菌属物种，包括11个最近描述的物种（阿尔本不动杆菌、波希米亚不动杆菌、耐黏菌素不动杆菌、库尔瓦利不动杆菌、分散不动杆菌、甘地不动杆菌、适度不动杆菌、蛋白水解不动杆菌、赛弗蒂不动杆菌、可变不动杆菌和维维安不动杆菌）以及所谓溶血分支的6个物种（拜耶林克不动杆菌、吉林伯格不动杆菌、溶血不动杆菌、琼氏不动杆菌、微小不动杆菌和威尼蒂亚努斯不动杆菌），接受了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析。使用市售布鲁克道尔顿公司的Biotyper数据库当前版本（8.0.0.0）评估鉴定结果，该数据库不包含所测试的6个物种的参考条目。高达29%的菌株被错误鉴定为Biotyper数据库中存在的不同不动杆菌属物种，这主要是由于溶血分支物种之间密切的系统发育关系。为了获得更可靠的鉴定结果，扩展商业数据库仅显示出部分改善，而使用替代的MALDI基质溶液（强酸化阿魏酸）几乎可以正确鉴定所有有问题的菌株。
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