Department of Clinical Laboratory, Henan Provincial People's Hospital, Henan University People's Hospital, Zhengzhou, 450003, Henan, China.
Henan Provincial People's Hospital, Henan University People's Hospital, and People's Hospital of Henan University, Weiwu Road 5, 450003, Zhengzhou, Henan, People's Republic of China.
BMC Microbiol. 2020 Oct 20;20(1):318. doi: 10.1186/s12866-020-02005-0.
To evaluate the accuracy and performance of the Autof MS1000 mass spectrometer in bacteria and yeast identification, 2342 isolates were obtained from microbial cultures of clinical specimens (e.g. blood, cerebrospinal fluid, respiratory tract samples, lumbar puncture fluid, wound samples, stool, and urine) collected in 2019 in Henan Provincial People's Hospital. Repetitive strains from the same patient were excluded. We tested the Autof MS1000 and Bruker Biotyper mass spectrometry systems and the classical biochemical identification system VITEK 2/API 20C AUX. Inconsistencies in strain identification among the three systems were identified by 16S rDNA and gene sequencing.
At the species level, the Autof MS1000 and Bruker Biotyper systems had isolate identification accuracies of 98.9 and 98.5%, respectively. At the genus level, the Autof MS1000 and Bruker Biotyper systems were 99.7 and 99.4% accurate, respectively. The instruments did not significantly differ in identification accuracy at either taxonomic level. The frequencies of unreliable identification were 1.1% (26/2342) for the Autof MS1000 and 1.5% (34/2342) for the Bruker Biotyper. In vitro experiments demonstrated that the coincidence rate of the Autof MS1000 mass spectrometer in the identification of five types of bacteria was > 93%, the identification error rate was < 3%, and the no identification rate was 0. This indicates that the Autof MS1000 system is acceptable for identification.
The Autof MS1000 mass spectrometer can be utilised to identify clinical isolates. However, an upgradation of the database is recommended to correctly identify rare strains.
为了评估 Autof MS1000 质谱仪在细菌和酵母鉴定中的准确性和性能，我们从 2019 年河南省人民医院采集的临床标本（如血液、脑脊液、呼吸道样本、腰椎穿刺液、伤口样本、粪便和尿液）的微生物培养物中获得了 2342 株分离株。排除了来自同一患者的重复菌株。我们测试了 Autof MS1000 和 Bruker Biotyper 质谱系统以及经典的生化鉴定系统 VITEK 2/API 20C AUX。通过 16S rDNA 和基因测序鉴定了三个系统之间菌株鉴定的不一致性。
在种水平上，Autof MS1000 和 Bruker Biotyper 系统的分离株鉴定准确率分别为 98.9%和 98.5%。在属水平上，Autof MS1000 和 Bruker Biotyper 系统的准确率分别为 99.7%和 99.4%。两种仪器在分类学水平上的鉴定准确率没有显著差异。Autof MS1000 的不可靠鉴定频率为 1.1%（26/2342），Bruker Biotyper 为 1.5%（34/2342）。体外实验表明，Autof MS1000 质谱仪对五种类型细菌的鉴定符合率>93%，鉴定错误率<3%，无鉴定率为 0。这表明 Autof MS1000 系统可用于鉴定。
Autof MS1000 质谱仪可用于鉴定临床分离株。然而，建议对数据库进行升级，以正确鉴定稀有菌株。
