De Simone V, Amati P
J Virol. 1987 May;61(5):1615-20. doi: 10.1128/JVI.61.5.1615-1620.1987.
To determine the relative growth advantages of polyomavirus regulatory region mutants selected from Friend leukemic and neuroblastoma cells persistently infected with polyomavirus A2 wild type, different murine cell lines, some of which are capable of further differentiation in vitro, were used as hosts in mixed infection and transfection. The tests allowed the determination, through the measurement of cis-advantage in replication, of the most effective polyomavirus regulatory region constitutions for a given cell line and, in some cases, for specific stages of cell differentiation. Furthermore, different domains of the viral regulatory region were shown to have different effects--positive, neutral, or negative--depending on the host analyzed.
为了确定从持续感染多瘤病毒A2野生型的Friend白血病细胞和神经母细胞瘤细胞中筛选出的多瘤病毒调控区突变体的相对生长优势，将不同的鼠细胞系（其中一些能够在体外进一步分化）用作混合感染和转染的宿主。通过测量复制中的顺式优势，这些试验能够确定对于给定细胞系以及某些情况下对于细胞分化的特定阶段而言最有效的多瘤病毒调控区组成。此外，病毒调控区的不同结构域根据所分析的宿主表现出不同的作用——正向、中性或负向。
