Bai Jianhui, Yan Weikai, Wang Yuqing, Yin Qiang, Liu Jinghui, Wight Charlene, Ma Baoluo
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
Experimental Station of Agricultural Ministry for Eco-environment Observation of Sandy Grassland in Ordos, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.
Front Plant Sci. 2018 Oct 2;9:1302. doi: 10.3389/fpls.2018.01302. eCollection 2018.
A set of four experiments was conducted to develop methods for screening oat tolerance to salt and alkali and the following results were obtained. (1) In experiment 1, 68.5 mmol L salt and 22.5 mmol L alkali were identified as appropriate concentrations for determining oat tolerance to salinity and alkalinity during germination. (2) These concentrations were used in experiment 2 to screen 248 oat genotypes and 21 were identified to be tolerant to salinity and alkalinity in germination. (3) In experiment 3, one salt treatment, 40 L of NaSO:NaCl (1:1), 150 mmol L, was found to be optimal for screening oat tolerance to salinity during growth and development. For alkalinity tolerance, the optimal treatment was 40 L of NaCO:NaHCO (1:1) at 75 mmol L. (4) No significant correlation was found between tolerances at the germination and adult stages or between tolerances to salt and alkali. Three lines were found to be tolerant to both salt and alkali in both germination and adult stages. (5) In experiment 4, 25 out of 262 oat genotypes were found to be tolerant to both salinity and alkalinity. (6) GGE biplot analysis was found to be effective in interpreting the multivariate data and the plastic cone-container system was found to be cost-effective system for screening adult plant tolerance to salt and alkali. (7) The symptoms of salt stress and alkali stress were found to be different; alkali stress mainly reduces the chlorophyll content, while salinity mainly disrupts water absorption.
进行了一组四个实验来开发筛选燕麦耐盐碱能力的方法,并获得了以下结果。(1)在实验1中,确定68.5 mmol/L的盐和22.5 mmol/L的碱为测定燕麦发芽期耐盐碱性的适宜浓度。(2)在实验2中使用这些浓度筛选了248个燕麦基因型,其中21个被鉴定为在发芽期耐盐碱性。(3)在实验3中,发现一种盐处理,即40 L的NaSO:NaCl(1:1),150 mmol/L,是筛选燕麦生长发育期间耐盐性的最佳处理。对于耐碱性,最佳处理是40 L的NaCO:NaHCO(1:1),75 mmol/L。(4)在发芽期和成年期的耐受性之间或对盐和碱的耐受性之间未发现显著相关性。发现有三个品系在发芽期和成年期均耐盐和碱。(5)在实验4中,发现262个燕麦基因型中有25个耐盐碱性。(6)发现GGE双标图分析在解释多变量数据方面有效,并且塑料锥形容器系统是筛选成年植物耐盐碱性的经济高效系统。(7)发现盐胁迫和碱胁迫的症状不同;碱胁迫主要降低叶绿素含量,而盐胁迫主要破坏水分吸收。