T细胞和B细胞的淋巴因子。I. 肿瘤抗原致敏小鼠的T细胞和B细胞产生改变靶细胞表面电荷的不同淋巴因子的证明。
Lymphokines of T and B cells. I. Demonstration that T and B cells of tumor antigensensitised mice produce different lymphokines changing surface charge of target cells.
作者信息
Jenssen H L, Eckert R, Werner H, Köhler H, Pasternak G, Friemel H
出版信息
Oncology. 1977;34(4):159-63. doi: 10.1159/000225213.
Lymphocytes from MC sarcoma-bearing mice were exposed to a soluble tumor antigen. The lymphokine-containing supernatant thus obtained was fractionated on Sephadex G-75. Fractions were tested for their activity to reduce the surface charge of indicator macrophages by cell electrophoresis technique. When unseparated spleen or lymph node cells were used the charge-reducing activity of the supernatant was found in three regions according to molecular weights of about 100,000, 60,000 - 35,000 and 13,000 Daltons. The appearance of activity was found to be time-dependent. As indicated by tests with separated lymphocytes both B and T cells appear to release charge-reducing activity.
将患有MC肉瘤的小鼠的淋巴细胞暴露于可溶性肿瘤抗原。由此获得的含淋巴因子的上清液在Sephadex G - 75上进行分级分离。通过细胞电泳技术测试各组分降低指示性巨噬细胞表面电荷的活性。当使用未分离的脾细胞或淋巴结细胞时，根据分子量约为100,000、60,000 - 35,000和13,000道尔顿，在上清液的三个区域发现了电荷降低活性。发现活性的出现与时间有关。如用分离的淋巴细胞进行的测试所示，B细胞和T细胞似乎都释放电荷降低活性。
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