Wahl S M, Wilton J M, Rosenstreich D L, Oppenheim J J
J Immunol. 1975 Apr;114(4):1296-301.
The role of macrophages in the production of two lymphokines, monocyte chemotactic factor and macrophage activating factor, was investigated. Lymphokine production by guinea pig lymph node and spleen cells required macrophages for thymus-dependent antigens and mitogens. In contrast, B cell stimulants which also induce the synthesis of lymphokines were macrophage independent. When populations of relatively pure B or T lymphocytes were isolated, it was found that T cells required viable macrophage cooperation to produce these two lymphokines and to undergo propliferation in response to specific antigens, whereas B cells could be directly activated in the absence of macrophages. These findings suggest that T and B cells have different requirements for activation and for macrophage cooperation. Futhermore, since lymphokine synthesis is evident within the first 4 hr of stimulant presentation, these observations demonstrate that macrophages play an essential role in the earliest events of lymphocyte activation.
研究了巨噬细胞在两种淋巴因子即单核细胞趋化因子和巨噬细胞激活因子产生过程中的作用。豚鼠淋巴结和脾细胞产生淋巴因子，对于胸腺依赖性抗原和有丝分裂原而言需要巨噬细胞参与。相比之下，同样能诱导淋巴因子合成的B细胞刺激剂则不依赖巨噬细胞。当分离出相对纯化的B淋巴细胞或T淋巴细胞群体时，发现T细胞需要有活力的巨噬细胞协同作用才能产生这两种淋巴因子，并在对特定抗原作出反应时进行增殖，而B细胞在没有巨噬细胞的情况下也能被直接激活。这些发现表明，T细胞和B细胞在激活以及巨噬细胞协同作用方面有不同的需求。此外，由于在刺激物呈现后的最初4小时内淋巴因子合成就很明显，这些观察结果表明巨噬细胞在淋巴细胞激活的最早事件中起关键作用。
