Ishida Juliane Karine, Caldas Danielle G G, Oliveira Lucas Roberto, Frederici Gabriela Campos, Leite Lucas Margato Pereira, Mui Tsai Siu
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo (CENA-USP), Piracicaba, SP, Brazil.
Genet Mol Biol. 2018 Oct-Dec;41(4):820-833. doi: 10.1590/1678-4685-GMB-2017-0272. Epub 2018 Oct 11.
Transporter proteins play an essential role in the uptake, trafficking and storage of metals in plant tissues. The Natural Resistance-Associated Macrophage Protein (NRAMP) family plays an essential role in divalent metal transport. We conducted bioinformatics approaches to identify seven NRAMP genes in the Phaseolus vulgaris genome, investigated their phylogenetic relation, and performed transmembrane domain and gene/protein structure analyses. We found that the NRAMP gene family forms two distinct groups. One group included the PvNRAMP1, -6, and -7 genes that share a fragmented structure with a numerous exon/intron organization and encode proteins with mitochondrial or plastidial localization. The other group is characterized by few exons that encode cytoplasmic proteins. In addition, our data indicated that PvNRAMP6 and -7 may be involved in mineral uptake and mobilization in nodule tissues, while the genes PvNRAMP1, -2, -3, -4 and -5 are potentially recruited during plant development. This data provided a more comprehensive understanding of the role of NRAMP transporters in metal homeostasis in P. vulgaris.
转运蛋白在植物组织中金属的吸收、运输和储存过程中发挥着至关重要的作用。天然抗性相关巨噬细胞蛋白(NRAMP)家族在二价金属转运中起着关键作用。我们采用生物信息学方法在菜豆基因组中鉴定出7个NRAMP基因,研究了它们的系统发育关系,并进行了跨膜结构域以及基因/蛋白质结构分析。我们发现NRAMP基因家族形成了两个不同的组。一组包括PvNRAMP1、-6和-7基因,它们具有片段化结构,外显子/内含子组织众多,编码定位于线粒体或质体的蛋白质。另一组的特点是外显子较少,编码细胞质蛋白。此外,我们的数据表明,PvNRAMP6和-7可能参与根瘤组织中的矿物质吸收和转运,而PvNRAMP1、-2、-3、-4和-5基因可能在植物发育过程中发挥作用。这些数据为更全面地了解NRAMP转运蛋白在菜豆金属稳态中的作用提供了依据。