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大豆（Glycine max (L.)）家族基因的全基因组鉴定与表达分析

Genome-Wide Identification and Expression Analysis of Family Genes in Soybean ( L.).

作者信息

Qin Lu, Han Peipei, Chen Liyu, Walk Thomas C, Li Yinshui, Hu Xiaojia, Xie Lihua, Liao Hong, Liao Xing

机构信息

Key Laboratory of Biology and Genetics Improvement of Oil Crops of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan, China.

Root Biology Center, Fujian Agriculture and Forestry UniversityFuzhou, China.

出版信息

Front Plant Sci. 2017 Aug 18;8:1436. doi: 10.3389/fpls.2017.01436. eCollection 2017.

DOI:10.3389/fpls.2017.01436
PMID:28868061
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5563376/
Abstract

The (natural resistance-associated macrophage protein) family of genes has been widely characterized in organisms ranging from bacteria to yeast, plants, mice, and humans. This gene family plays vital roles in divalent metal ion transport across cellular membranes. As yet, comprehensive analysis of family genes has not been reported for soybean. In this study, bioinformatics analysis was conducted to identify 13 soybean genes, along with their gene structures, phylogenetic relationships, and transmembrane domains. Expression analysis suggests that genes function in numerous tissues and development stages. Moreover, soybean genes were differentially regulated by deficiencies of N, P, K, Fe, and S, along with toxicities of Fe, Cu, Cd, and Mn. These results indicate that genes function in many nutrient stress pathways, and might be involved in crosstalk among nutrient stress pathways. Subcellular localization analysis in Arabidopsis protoplasts confirmed the tonoplast or plasma membrane localization of selected soybean NRMAP proteins. Protein-protein interaction analysis found that the networks of three GmNRAMP proteins which putatively interact with nodulin-like proteins, almost distinct from the network that is common to the other 10 soybean NRAMP proteins. Subsequent qRT-PCR results confirmed that these three genes exhibited enhanced expression in soybean nodules, suggesting potential functions in the transport of Fe or other metal ions in soybean nodules. Overall, the systematic analysis of the gene family reported herein provides valuable information for further studies on the biological roles of in divalent metal ion transport in various soybean tissues under numerous nutrient stresses and soybean-rhizobia symbiosis.

摘要

（天然抗性相关巨噬细胞蛋白）基因家族在从细菌到酵母、植物、小鼠和人类等多种生物体中已得到广泛表征。该基因家族在二价金属离子跨细胞膜运输中起着至关重要的作用。迄今为止，尚未见关于大豆中该基因家族的全面分析报道。在本研究中，进行了生物信息学分析以鉴定13个大豆该基因，以及它们的基因结构、系统发育关系和跨膜结构域。表达分析表明该基因在众多组织和发育阶段发挥作用。此外，大豆该基因受氮、磷、钾、铁和硫缺乏以及铁、铜、镉和锰毒性的差异调节。这些结果表明该基因在许多营养胁迫途径中发挥作用，并且可能参与营养胁迫途径之间的相互作用。在拟南芥原生质体中的亚细胞定位分析证实了所选大豆NRMAP蛋白定位于液泡膜或质膜。蛋白质-蛋白质相互作用分析发现，三种推测与类结瘤蛋白相互作用的GmNRAMP蛋白的网络，几乎与其他10种大豆NRAMP蛋白共有的网络不同。随后的qRT-PCR结果证实，这三个该基因在大豆根瘤中表达增强，表明它们在大豆根瘤中铁或其他金属离子运输中具有潜在功能。总体而言，本文报道的对该基因家族的系统分析为进一步研究该基因在多种营养胁迫下大豆不同组织以及大豆-根瘤菌共生中二价金属离子运输的生物学作用提供了有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/4dadc8342e13/fpls-08-01436-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/ea78a87e58bb/fpls-08-01436-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/82f306227dc5/fpls-08-01436-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/1e03e698273a/fpls-08-01436-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/109fdfbf057d/fpls-08-01436-g0007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/4dadc8342e13/fpls-08-01436-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/ea78a87e58bb/fpls-08-01436-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/131bbc39d4a2/fpls-08-01436-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/7d5aea82489a/fpls-08-01436-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/adee3bcb6f05/fpls-08-01436-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/82f306227dc5/fpls-08-01436-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/1e03e698273a/fpls-08-01436-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/109fdfbf057d/fpls-08-01436-g0007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70f4/5563376/4dadc8342e13/fpls-08-01436-g0009.jpg

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