State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , Nanjing 210096 , China.
ACS Appl Mater Interfaces. 2018 Nov 14;10(45):39144-39150. doi: 10.1021/acsami.8b11552. Epub 2018 Oct 30.
Here, we propose a new platform for digital nucleic acid assay based on a microdroplet array. The hydrophilic-superhydrophobic property of the pseudopaper photonic nitrocellulose substrate is employed to divide an aqueous sample containing the target nucleic acid into hundreds of microdroplets for loop-mediated isothermal amplification (LAMP). The LAMP reaction leads to the production of fluorescent calcein. By counting the number of microdroplets that are fluorescent, the target nucleic acid (i.e., Staphylococcus aureus DNA) with the copy number ranging from 1 to 1000 can be absolutely quantified without using sophisticated microfluidic devices. The effect of fluorescence enhancement attributed to the photonic nitrocellulose can effectively amplify the detection signal and reduce the amplification time. Thus, we believe that the proposed platform for digital nucleic acid assay based on the microdroplet array is promising for rapid and cost-effective bioanalytical applications.
在这里，我们提出了一种基于微滴阵列的新型数字核酸检测平台。利用伪纸状光学生物适体的亲水-超疏水性质，将含有靶核酸的水性样品分成数百个微滴，用于环介导等温扩增（LAMP）。LAMP 反应导致荧光钙黄绿素的产生。通过计数荧光微滴的数量，可以绝对定量靶核酸（即金黄色葡萄球菌 DNA）的拷贝数范围从 1 到 1000，而无需使用复杂的微流控设备。光学生物适体的荧光增强效应可以有效放大检测信号并缩短扩增时间。因此，我们相信，基于微滴阵列的数字核酸检测平台具有快速、经济高效的生物分析应用前景。
