Nagashima Miki, Koyanagi Madoka, Arimura Yutaka
a Host Defense for Animals , Nippon Veterinary and Life Science University , Musashino-shi, Tokyo , Japan.
Immunol Invest. 2019 Apr;48(3):303-320. doi: 10.1080/08820139.2018.1523924. Epub 2018 Oct 18.
Allergic diseases have increased in the last three decades. Mast cells play a critical role in allergic diseases along with allergen-specific immunoglobulin E (IgE). Following mast cell degranulation elicited by ligation of the IgE-FcεRI receptor complex with allergen, allergic reactions are followed by various symptoms such as vascular hyperpermeability, mucous secretion, itching, sneezing, wheezing, rashes, fever, and anaphylactic shock. Susceptibility or inclination to allergy varies depending on individual genetic traits and living environment, and it has long been believed that such an inclination is determined by an immunologic balance of T helper cell types. Mouse strains also have different susceptibilities to allergy. Similar to T helper cells and macrophages, it is not known whether mast cells can also be divided into two different types between mouse strains. In this study, we prepared bone marrow-derived mast cells from BALB/c and C57BL/6 mice and examined their cellular properties. Cellular response to IL-3 and the process of mast cell differentiation from bone marrow cells were different on the basis of cell surface marker molecules. BALB/c-derived cells more efficiently exhibited degranulation than did C57BL/6-derived cells following both calcium ionophore and receptor crosslinking. These functional differences persisted even after a longer cell culture for 8 weeks, suggesting a difference in cell-autonomous characteristics. These results support the concept that mast cells also have different cell types dependent on their genetic background. Abbreviations: Ab: antibody; BMMC: bone marrow-derived mast cell; DNP: dinitrophenyl; FACS: fluorescence-activated cell sorter; FCS: fetal calf serum; FITC: fluorescein isothiocyanate; FSC: forward scatter; HRP: horseradish peroxidase; HSA: human serum albumin; Ig: immunoglobulin; IL: interleukin; MIP-2: macrophage inflammatory protein-2; MCP: mast cell protease; PE: phycoerythrin; PerCP: Peridinin chlorophyll protein complex; SNP: single nucleotide polymorphisms; SSC: side scatter; Th: T helper; TNF-α: tumor necrosis factor alpha.
在过去三十年中，过敏性疾病有所增加。肥大细胞与过敏原特异性免疫球蛋白E（IgE）一起在过敏性疾病中起关键作用。在IgE-FcεRI受体复合物与过敏原连接引发肥大细胞脱颗粒后，过敏反应会伴随各种症状，如血管通透性增加、黏液分泌、瘙痒、打喷嚏、喘息、皮疹、发热和过敏性休克。过敏的易感性或倾向因个体遗传特征和生活环境而异，长期以来人们一直认为这种倾向是由辅助性T细胞类型的免疫平衡决定的。小鼠品系对过敏也有不同的易感性。与辅助性T细胞和巨噬细胞类似，尚不清楚在小鼠品系之间肥大细胞是否也可分为两种不同类型。在本研究中，我们从BALB/c和C57BL/6小鼠制备了骨髓来源的肥大细胞，并检测了它们的细胞特性。基于细胞表面标志物分子，细胞对白细胞介素-3的反应以及骨髓细胞向肥大细胞的分化过程有所不同。在钙离子载体和受体交联后，源自BALB/c的细胞比源自C57BL/6的细胞更有效地表现出脱颗粒。即使在较长时间（8周）的细胞培养后，这些功能差异仍然存在，表明细胞自主特性存在差异。这些结果支持了肥大细胞也因其遗传背景而具有不同细胞类型的概念。缩写：Ab：抗体；BMMC：骨髓来源的肥大细胞；DNP：二硝基苯基；FACS：荧光激活细胞分选仪；FCS：胎牛血清；FITC：异硫氰酸荧光素；FSC：前向散射；HRP：辣根过氧化物酶；HSA：人血清白蛋白；Ig：免疫球蛋白；IL：白细胞介素；MIP-2：巨噬细胞炎性蛋白-2；MCP：肥大细胞蛋白酶；PE：藻红蛋白；PerCP：多甲藻叶绿素蛋白复合物；SNP：单核苷酸多态性；SSC：侧向散射；Th：辅助性T细胞；TNF-α：肿瘤坏死因子α
