通过部分填充II型限制性核酸内切酶产生的DNA的5'-末端来提高克隆选择性的交叉索引。
Cross index for improving cloning selectivity by partially filling in 5'-extensions of DNA produced by type II restriction endonucleases.
作者信息
Korch C
出版信息
Nucleic Acids Res. 1987 Apr 24;15(8):3199-220. doi: 10.1093/nar/15.8.3199.
Abstract
A cross index is presented for using the improved selectivity offered by the Hung and Wensink (Nucl. Acids Res. 12, 1863-1874, 1984) method of partially filling in 5'-extensions produced by type II restriction endonucleases. After this treatment, DNA fragments which normally cannot be ligated to one another, can be joined providing that complementary cohesive ends have been generated. The uses of this technique, which include the prevention of DNA fragments (both vector and insert) auto-annealing, are discussed.
摘要
本文提供了一个交叉索引，用于利用Hung和Wensink（《核酸研究》，12卷，1863 - 1874页，1984年）方法所提供的更高选择性，该方法用于部分填充由II型限制性内切核酸酶产生的5'端延伸。经过这种处理后，通常不能彼此连接的DNA片段，只要产生了互补的粘性末端，就可以连接在一起。本文还讨论了该技术的用途，包括防止DNA片段（载体和插入片段）自身退火。
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本文引用的文献
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