通过质子核磁共振比较含有中心G-T碱基对的寡核苷酸与非错配序列的构象。
Comparison of the conformation of an oligonucleotide containing a central G-T base pair with the non-mismatch sequence by proton NMR.
作者信息
Quignard E, Fazakerley G V, van der Marel G, van Boom J H, Guschlbauer W
出版信息
Nucleic Acids Res. 1987 Apr 24;15(8):3397-409. doi: 10.1093/nar/15.8.3397.
Abstract
We have recorded NOESY spectra of two non-selfcomplementary undecanucleotide duplexes. From the observed NOEs we do not detect any significant distortion of the helix when a G-C pair is replaced by a G-T pair and the normal interresidue connectivities can be followed through the mismatch site. We conclude that the 2D spectra of the non-exchangeable protons do not allow differentiation between a wobble or rare tautomer form for the mismatch. NOE measurements in H2O, however, clearly show that the mismatch adopts a wobble structure and give information on the hydration in the minor groove for the G-T base pair which is embedded between two A-T base pairs in the sequence.
摘要
我们记录了两种非自互补的十一核苷酸双链体的NOESY谱。从观察到的核Overhauser效应（NOE）中，当一个G-C碱基对被一个G-T碱基对取代时，我们未检测到螺旋有任何明显扭曲，并且正常的残基间连接性可以穿过错配位点。我们得出结论，不可交换质子的二维谱不允许区分错配的摆动或罕见互变异构形式。然而，在H₂O中的NOE测量清楚地表明，错配采用摆动结构，并给出了在该序列中嵌入在两个A-T碱基对之间的G-T碱基对小沟中水合作用的信息。
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